Home / CXCR2/IL-8RB / Mouse CXCR2/IL-8RB APC-conjugated Antibody

Mouse CXCR2/IL-8RB APC-conjugated Antibody

Catalog #: FAB2164A
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2164A-025
FAB2164A-100
Detection of CXCR2/IL‑8 RB in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCR2/IL-8RB APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Stains mouse CXCR2-transfected cells but not CXCR1 transfectants.
Source
Monoclonal Rat IgG2A Clone # 242216
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C6 rat glioma cell line transfected with mouse CXCR2
Met1-Leu359
Accession # P35343
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR2/IL-8 RB antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CXCR2/IL‑8 RB in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F) and either (A) Rat Anti-Mouse CXCR2/IL-8 RB APC-conjugated Monoclonal Antibody (Catalog # FAB2164A) or (B) Rat IgG1Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CXCR2/IL-8 RB antibody in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP antibody by Flow Cytometry. View Larger

Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL-8 RB APC-conjugated Monoclonal Antibody (Catalog # FAB2164A). Quadrant markers were set based on control antibody staining (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR2/IL-8RB

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

Long Name
Interleukin 8 Receptor B
Entrez Gene IDs
3579 (Human); 12765 (Mouse); 29385 (Rat)
Alternate Names
CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; CMKAR2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 RB; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B

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Citations for Mouse CXCR2/IL-8RB APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. E-selectin-mediated rapid NLRP3 inflammasome activation regulates S100A8/S100A9 release from neutrophils via transient gasdermin D pore formation
    Authors: Pruenster, M;Immler, R;Roth, J;Kuchler, T;Bromberger, T;Napoli, M;Nussbaumer, K;Rohwedder, I;Wackerbarth, LM;Piantoni, C;Hennis, K;Fink, D;Kallabis, S;Schroll, T;Masgrau-Alsina, S;Budke, A;Liu, W;Vestweber, D;Wahl-Schott, C;Roth, J;Meissner, F;Moser, M;Vogl, T;Hornung, V;Broz, P;Sperandio, M;
    Nature immunology
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Rac GTPase Activating Protein ARHGAP25 Regulates Leukocyte Transendothelial Migration in Mice
    J Immunol, 2016-08-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CXCR1/2 inhibition blocks and reverses type 1 diabetes in mice.
    Authors: Citro A, Valle A, Cantarelli E, Mercalli A, Pellegrini S, Liberati D, Daffonchio L, Kastsiuchenka O, Ruffini P, Battaglia M, Allegretti M, Piemonti L
    Diabetes, 2014-10-14;64(4):1329-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
    EMBO J., 2012-07-31;31(17):3607-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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