Mouse Coagulation Factor III/Tissue Factor Biotinylated Antibody

Catalog #: BAF3178 Datasheet / COA / SDS

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BAF3178

All Coagulation Factor III/Tissue Factor Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Coagulation Factor III/Tissue Factor Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Coagulation Factor III/Tissue Factor in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human TF is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Coagulation Factor III/Tissue Factor
Ala29-Glu251
Accession # P20352

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Coagulation Factor III/Tissue Factor (Catalog # 3178-PA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-294) (3, 4). The purified recombinant mouse TF corresponds to the ectodomain (residues 29-251) and is potent in activating thermolysin-processed Recombinant Mouse Coagulation Factor VII (R&D Systems, Catalog # 3305-SE) under the conditions described in the Activity Assay Protocol.

References

Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) Academic Press, San Diego, p. 1659.
Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
Ranganathan, G. et al. (1991) J. Biol. Chem. 266:496.
Hartzell, S. (1989) Mol. Cell. Biol. 9:2567.

Entrez Gene IDs

2152 (Human); 14066 (Mouse)

Alternate Names

CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor