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Mouse Chitinase 3-like 1/YKL-40 Antibody

Catalog #: AF2649
3 Citations Datasheet / COA / SDS
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AF2649
AF2649-SP
Detection of Mouse Chitinase 3‑like 1/YKL-40 by Western Blot.
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Citations (3)
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Mouse Chitinase 3-like 1/YKL-40 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Chitinase 3-like 1/YKL-40 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human Chitinase 3-like 1/YKL-40 is observed.
Source
Polyclonal Sheep IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Lysates of mouse bone marrow and THP‑1 human acute monocytic leukemia cell line, THP‑1 human acute monocytic leukemia cell line + PMA/LPS.
Simple Western
25 µg/mL
Lysates of mouse bone marrow and THP-1 human acute monocytic leukemia cell line +/- PMA/LPS
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Chitinase 3-like 1/YKL-40 (Catalog # 2649-CH), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Mouse Chitinase 3‑like 1/YKL-40 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, THP‑1 human acute monocytic leukemia cell line + PMA/LPS untreated (-) or treated (+) with 200 nm, 10 μg/ml PMA and LPS for 24hr, 3hr. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Chitinase 3‑like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2649) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Western Blot View Larger

Detection of Mouse Chitinase 3‑like 1/YKL-40 by Western Blot. Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Chitinase 3‑like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2649) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western View Larger

Detection of Mouse Chitinase 3‑like 1/YKL-40 by Simple WesternTM. Simple Western lane view shows lysates of Mouse bone marrow, loaded at 0.2 mg/mL. A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 50 kDa (as indicated) using 25 µg/mL of Sheep Anti-Mouse Chitinase 3‑like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2649). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western View Larger

Detection of Mouse Chitinase 3‑like 1/YKL-40 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA for 24 hours and 10 μg/ml LPS for 3 hours, loaded at 0.2 mg/mL. A specific band was detected for Chitinase 3‑like 1/YKL-40 at approximately 50 kDa (as indicated) using 25 µg/mL of Sheep Anti-Mouse Chitinase 3‑like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2649). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1 (CHI3L1), also called breast regression protein 39 (BRP39) in mouse, or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5-7). The mouse CHI3L1 cDNA encodes 381 amino acids (aa), including a 21 aa signal sequence and a 360 aa mature region with two intermolecular disulfides (3). Mature mouse CHI3L1 shares 73%, 75%, 72%, 71%, 70% and 69% aa sequence identity with human, rat, equine, porcine, canine and bovine CHI3L1, respectively. CHI3L1 does not show chitotriosidase activity, but binds chitin and is thus termed a chi-lectin (1-4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Human elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Human single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15). Mice lacking CHI3L1 have markedly diminished antigen-induced Th2 responses, which can be rescued by expression of human CHI3L1 (16).

References
  1. Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
  2. Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
  3. Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
  4. Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
  5. Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
  6. Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
  7. Shao, R. et al. (2009) Oncogene 28:4456.
  8. Recklies, A.D. et al. (2002) Biochem. J. 365:119.
  9. Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
  10. Letuve, S. et al. (2008) J Immunol. 181:5167.
  11. Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
  12. Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
  13. Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
  14. Johansen, J.S. et al. (1999) Rheumatology 38:618.
  15. Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.
  16. Lee, C.G. et al. (2009) J. Exp. Med. 206:1149.
Entrez Gene IDs
1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)
Alternate Names
AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40

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Product Specific Notices

This product or the use of this product is covered by US Patent # 7,230,086 which is owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes.

Citations for Mouse Chitinase 3-like 1/YKL-40 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Activated Neutrophils Secrete Chitinase-Like 1 and Attenuate Liver Inflammation by Inhibiting Pro-Inflammatory Macrophage Responses
    Authors: Yu Lu, Na Chang, Xinhao Zhao, Renmin Xue, Jing Liu, Lin Yang et al.
    Frontiers in Immunology
  2. Chitinase 3‐like 1 protein plays a critical role in respiratory syncytial virus‐induced airway inflammation
    Authors: Min Jung Kim, Doo Hee Shim, Hye‐Ran Cha, Kuk‐Young Moon, Chang Mo Yang, Su Jin Hwang et al.
    Allergy
  3. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
    Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
    Biomark Insights, 2012-07-10;7(0):87-104.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot

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