Home / Chitinase 3-like 1/YKL-40 / Mouse Chitinase 3-like 1/YKL-40 Antibody

Mouse Chitinase 3-like 1/YKL-40 Antibody

Catalog #: MAB2649
7 Citations Datasheet / COA / SDS
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MAB2649
MAB2649-SP
Detection of Mouse Chitinase 3‑like 1/YKL-40 by Western Blot.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Chitinase 3-like 1/YKL-40 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Chitinase 3-like 1/YKL-40 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human Chitinase 3-like 1/YKL-40.
Source
Monoclonal Rat IgG2B Clone # 321924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Chitinase 3-like 1/YKL-40
Tyr22-Ala381
Accession # Q61362
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Chitinase 3-like 1 antibody by Western Blot. View Larger

Detection of Mouse Chitinase 3‑like 1/YKL-40 by Western Blot. Western blot shows lysates of mouse lung tissue and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Chitinase 3-like 1/YKL-40 Monoclonal Antibody (Catalog # MAB2649) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for Chitinase 3-like 1/YKL-40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1 (CHI3L1), also called breast regression protein 39 (BRP39) in mouse, or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5-7). The mouse CHI3L1 cDNA encodes 381 amino acids (aa), including a 21 aa signal sequence and a 360 aa mature region with two intermolecular disulfides (3). Mature mouse CHI3L1 shares 73%, 75%, 72%, 71%, 70% and 69% aa sequence identity with human, rat, equine, porcine, canine and bovine CHI3L1, respectively. CHI3L1 does not show chitotriosidase activity, but binds chitin and is thus termed a chi-lectin (1-4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Human elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Human single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15). Mice lacking CHI3L1 have markedly diminished antigen-induced Th2 responses, which can be rescued by expression of human CHI3L1 (16).

References
  1. Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
  2. Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
  3. Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
  4. Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
  5. Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
  6. Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
  7. Shao, R. et al. (2009) Oncogene 28:4456.
  8. Recklies, A.D. et al. (2002) Biochem. J. 365:119.
  9. Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
  10. Letuve, S. et al. (2008) J Immunol. 181:5167.
  11. Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
  12. Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
  13. Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
  14. Johansen, J.S. et al. (1999) Rheumatology 38:618.
  15. Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.
  16. Lee, C.G. et al. (2009) J. Exp. Med. 206:1149.
Entrez Gene IDs
1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)
Alternate Names
AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40

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Citations for Mouse Chitinase 3-like 1/YKL-40 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Conditional deletion of Stat3 in mammary epithelium impairs the acute phase response and modulates immune cell numbers during post‐lactational regression
    Authors: Katherine Hughes, Julie A. Wickenden, Judith E. Allen, Christine J. Watson
    The Journal of Pathology
  2. Chitinase 3-like 1 plays a pivotal role in airway response of RSV infection via regulating DC functional transition
    Authors: Ge, L;Wang, Y;Liu, Z;Du, H;Zhao, D;
    International immunopharmacology
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Age-related changes in brain phospholipids and bioactive lipids in the APP knock-in mouse model of Alzheimer's disease
    Authors: C Emre, KV Do, B Jun, E Hjorth, SG Alcalde, MI Kautzmann, WC Gordon, P Nilsson, NG Bazan, M Schultzber
    Acta neuropathologica communications, 2021-06-29;9(1):116.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis
    Authors: DH Kim, HJ Park, S Lim, JH Koo, HG Lee, JO Choi, JH Oh, SJ Ha, MJ Kang, CM Lee, CG Lee, JA Elias, JM Choi
    Nat Commun, 2018-02-05;9(1):503.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Stat3 downstream gene product chitinase 3-like 1 is a potential biomarker of inflammation-induced lung cancer in multiple mouse lung tumor models and humans.
    Authors: Yan, Cong, Ding, Xinchun, Wu, Lingyan, Yu, Menggang, Qu, Peng, Du, Hong
    PLoS ONE, 2013-04-22;8(4):e61984.
    Species: Mouse
    Sample Types: BALF, Whole Tissue
    Applications: IHC, Western Blot
  6. Breast regression protein-39 (BRP-39) promotes dendritic cell maturation in vitro and enhances Th2 inflammation in murine model of asthma.
    Authors: Xu Q, Chai S, Qian Y, Zhang M, Wang K
    Acta Pharmacol Sin, 2012-11-26;33(12):1525-32.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Chitinase-Like Proteins Are Autoantigens in a Model of Inflammation-Promoted Incipient Neoplasia
    Authors: Asif M Qureshi, Adele Hannigan, Donald Campbell, Colin Nixon, Joanna B Wilson
    Genes & Cancer

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