Home / Cerberus 1 / Mouse Cerberus 1 Antibody

Mouse Cerberus 1 Antibody

Catalog #: AF1986
3 Citations Datasheet / COA / SDS
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AF1986
AF1986-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cerberus 1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cerberus 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human Cerberus is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cerberus 1
Asp41-Pro272
Accession # O55233
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Cerberus 1 (Catalog # 1986-CB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cerberus 1

Cerberus 1, also called DAND4, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and COCO/Dante (DAND5). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1, 2). Mature mouse Cerberus 1 shares 40%, 29%, and 66% amino acid (aa) sequence identity with chick, Xenopus, and human Cerberus 1, respectively. Within the cysteine-knot domain, it shares 24%‑37% aa sequence identity with mouse DAN, Gremlin, PRDC, and COCO. Cerberus 1 is a secreted 38 kDa glycoprotein that forms disulfide-linked homodimers (3). Cerberus-S, which is generated by proteolysis in Xenopus, is a short version of the molecule and includes the C-terminal cysteine-knot domain (4). At the onset of gastrulation, Cerberus 1 is transiently expressed in anterior endodermal structures in response to Nodal and Shh (3, 5‑9). Cerberus 1 binds BMP-4 and Nodal and inhibits their activities. Xenopus Cerberus has also been shown to bind Xenopus Wnt8. These inhibitory functions of Cerberus favor mesodermal development in the anterior region of the gastrula and suppresses posterior mesodermal differentiation (3, 4, 6, 8, 10‑12). In chick and Xenopus, Cerberus 1 also regulates, but is not required for embryonic left-right polarization, neurulation, and head and heart induction (4‑8, 13).

References
  1. Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
  2. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
  3. Biben, C. et al. (1998) Dev. Biol. 194:135.
  4. Piccolo, S. et al. (1999) Nature 397:707.
  5. Zhu, L. et al. (1999) Curr. Biol. 9:931.
  6. Tavares, A.T. et al. (2007) Development 134:2051.
  7. Foley, A.C. et al. (2007) Dev. Biol. 303:57.
  8. Bouwmeester, T. et al. (1996) Nature 382:595.
  9. Bertocchini, F. and C.D. Stern (2002) Dev. Cell 3:735.
  10. Glinka, A. et al. (1997) Nature 389:517.
  11. Perea-Gomez, A. et al. (2002) Dev. Cell 3:745.
  12. Takaoka, K. et al. (2007) Curr. Opin. Genet. Dev. 17:344
  13. Simpson, E.H. et al. (1999) Dev. Biol. 213:202.
Entrez Gene IDs
9350 (Human); 12622 (Mouse)
Alternate Names
CER1; cerberus 1 (Xenopus laevis) homolog (cysteine knot superfamily); Cerberus 1; cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis); cerberus; cerberus-related 1; Cerberus-related protein; DAN domain family member 4; DAND4; MGC119894; MGC119895; MGC96951

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Citations for Mouse Cerberus 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. A simple and robust method for establishing homogeneous mouse epiblast stem cell lines by wnt inhibition.
    Authors: Sugimoto M, Kondo M, Koga Y, Shiura H, Ikeda R, Hirose M, Ogura A, Murakami A, Yoshiki A, Chuva de Sousa Lopes S, Abe K
    Stem Cell Reports, 2015-03-26;4(4):744-57.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Secreted Cerberus1 as a Marker for Quantification of Definitive Endoderm Differentiation of the Pluripotent Stem Cells
    Authors: Hidefumi Iwashita, Nobuaki Shiraki, Daisuke Sakano, Takashi Ikegami, Masanobu Shiga, Kazuhiko Kume et al.
    PLoS ONE
  3. Genome-wide haplotype association mapping in mice identifies a genetic variant in CER1 associated with BMD and fracture in southern Chinese women.
    Authors: Tang PL, Cheung CL, Sham PC, McClurg P, Lee B, Chan SY, Smith DK, Tanner JA, Su AI, Cheah KS, Kung AW, Song YQ
    J. Bone Miner. Res., 2009-06-01;24(6):1013-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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