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Mouse CD69 Antibody

Catalog #: AF2386
5 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2386
AF2386-SP
Detection of CD69 in mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight vs unstimulated mouse splenocytes by Flow Cytometry
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Citations (5)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD69 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross‑reactivity with recombinant human CD69 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD69
Gly64-Arg199
Accession # P37217
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD69
Flow Cytometry
0.25 µg/106 cells
- CTLL‑2 mouse cytotoxic T cell line
- Mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight vs unstimulated mouse splenocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of CD69 in mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight vs unstimulated mouse splenocytes by Flow Cytometry Mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight (A) vs unstimulated mouse splenocytes (B) were stained with Goat Anti-Mouse CD69 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2386) and Rat Anti-Mouse CD3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4841F) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells and platelets. The C-terminal extracellular domain of mouse CD69 shares approximately 57% amino acid sequence homology with the corresponding region of human CD69.

Entrez Gene IDs
969 (Human); 12515 (Mouse)
Alternate Names
activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60

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Citations for Mouse CD69 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Lymph node conduits transport virions for rapid T cell activation
    Authors: Glennys V. Reynoso, Andrea S. Weisberg, John P. Shannon, Daniel T. McManus, Lucas Shores, Jeffrey L. Americo et al.
    Nature Immunology
  2. CD8+ T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging
    Authors: T Kaya, N Mattugini, L Liu, H Ji, L Cantuti-Ca, J Wu, M Schifferer, J Groh, R Martini, S Besson-Gir, S Kaji, A Liesz, O Gokce, M Simons
    Nature Neuroscience, 2022-10-24;25(11):1446-1457.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Differentiation Paths of Peyer's Patch LysoDCs Are Linked to Sampling Site Positioning, Migration, and T Cell Priming
    Authors: C Wagner, J Bonnardel, C Da Silva, L Spinelli, CA Portilla, J Tomas, M Lagier, L Chasson, M Masse, M Dalod, A Chollat-Na, JP Gorvel, H Lelouard
    Cell Rep, 2020-04-07;31(1):107479.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Dynamic Reorganization of Chromatin Accessibility Signatures during Dedifferentiation of Secretory Precursors into Lgr5+ Intestinal Stem Cells
    Authors: U Jadhav, M Saxena, NK O'Neill, A Saadatpour, GC Yuan, Z Herbert, K Murata, RA Shivdasani
    Cell Stem Cell, 2017-06-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. CXCR3 chemokine receptor enables local CD8(+) T cell migration for the destruction of virus-infected cells.
    Authors: Hickman H, Reynoso G, Ngudiankama B, Cush S, Gibbs J, Bennink J, Yewdell J
    Immunity, 2015-03-10;42(3):524-37.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Mouse CD69 Antibody
By Efstathios Stamatiades on 07/09/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Mammary gland tissue Species: Mouse

Cryosections from PLP immersion fixed PyMT mammary gland tumors were stained for CD69 (1:100) for 4h @ RT. After washing, tissue was stained with Donkey anti-Goat alexa546 2ndary antibody for 1h at RT. CD3 in green, CD69 in red, nuclear staining (Hoechst) in blue.

Immunocytochemistry/Immunofluorescence Mouse CD69 Antibody AF2386