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Mouse CD4 Antibody

Catalog #: MAB554
17 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB554-SP
MAB554-100
MAB554-500
CD4 in Mouse Intestine.
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Product Details
Citations (17)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD4 Antibody Summary

Species Reactivity
Mouse
Specificity
Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.
Source
Monoclonal Rat IgG2B Clone # GK1.5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse CTL clone V4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
Immunohistochemistry
1-25 µg/mL
See below
Immunoprecipitation
Dialynas, D.P. et al. (1983) J. Immunol. 131:2445.
 
Cell depletion
Dialynas, D.P. et al. (1983) Immunol. Rev. 74:29; Martin, P. et al. (2000) Blood 96:2511.
 
Costimulation of T Cells
Bosselut, R. et al. (1999) J. Exp. Med. 190:1517.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Inhibition of T Cell Function
Dialynas, D.P. et al. (1983) J. Immunol. 131:2445; Dialynas, D.P. et al. (1983) Immunol. Rev. 74:29; Kruman, I.I. et al. (1996) Cell. Immunol. 173:236; Grakoui, A. et al. (1999) Science 285:221.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CD4 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr). View Larger

CD4 in Mouse Intestine. CD4 was detected in immersion fixed frozen sections of mouse intestine using Rat Anti-Mouse CD4 Monoclonal Antibody (Catalog # MAB554) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names
CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Citations for Mouse CD4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Eggerthella lenta augments preclinical autoantibody production and metabolic shift mimicking senescence in arthritis
    Authors: Balakrishnan, B;Luckey, D;Wright, K;Davis, JM;Chen, J;Taneja, V;
    Science advances
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. CD4+ T cells aggravate hemorrhagic brain injury
    Authors: Shi, SX;Xiu, Y;Li, Y;Yuan, M;Shi, K;Liu, Q;Wang, X;Jin, WN;
    Science advances
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Survival of stem cells and progenitors in the intestine is regulated by LPA5-dependent signaling
    Authors: Z Liang, P He, Y Han, CC Yun
    Cellular and Molecular Gastroenterology and Hepatology, 2022-04-04;0(0):.
    Species: Mouse
    Sample Types: Tissue, Whole Cells
    Applications: ICC/IF, IHC
  4. Alzheimer's disease protease-containing plasma extracellular vesicles transfer to the hippocampus via the choroid plexus
    Authors: JH Lee, C Ostalecki, T Oberstein, S Schierer, E Zinser, M Eberhardt, K Blume, B Plosnita, L Stich, H Bruns, R Coras, J Vera-Gonza, M Maler, AS Baur
    EBioMedicine, 2022-02-25;77(0):103903.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. RNU (Foxn1 RNU-Nude) Rats Demonstrate an Improved Ability to Regenerate Muscle in a Volumetric Muscle Injury Compared to Sprague Dawley Rats
    Authors: Michael J. McClure, Lucas C. Olson, David J. Cohen, Yen Chen Huang, Shirley Zhang, Tri Nguyen et al.
    Bioengineering (Basel)
  6. Chronic mild hypoxia accelerates recovery from preexisting EAE by enhancing vascular integrity and apoptosis of infiltrated monocytes
    Authors: SK Halder, R Milner
    Proc. Natl. Acad. Sci. U.S.A., 2020-05-05;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Interleukin-12p35 Deficiency Reverses the Th1/Th2 Imbalance, Aggravates the Th17/Treg Imbalance, and Ameliorates Atherosclerosis in ApoE-/- Mice
    Authors: Y Huang, H Hu, L Liu, J Ye, Z Wang, B Que, W Liu, Y Shi, T Zeng, L Shi, Q Ji, C Chang, Y Lin
    Mediators Inflamm., 2019-04-10;2019(0):3152040.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4
    Authors: Mudan Zhang, Xu Deng, Xinmeng Guan, Lanlan Geng, Ming Fu, Binman Zhang et al.
    Frontiers in Immunology
  9. Hypoxic pre-conditioning suppresses experimental autoimmune encephalomyelitis by modifying multiple properties of blood vessels
    Authors: SK Halder, R Kant, R Milner
    Acta Neuropathol Commun, 2018-09-03;6(1):86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Combination of CD40 agonism and CSF-1R blockade reconditions tumor-associated macrophages and drives potent antitumor immunity
    Authors: KR Wiehagen, NM Girgis, DH Yamada, AA Smith, SR Chan, IS Grewal, M Quigley, RI Verona
    Cancer Immunol Res, 2017-11-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. The efficacy of combination therapy with oncolytic herpes simplex virus HF10 and dacarbazine in a mouse melanoma model
    Authors: R Tanaka, F Goshima, S Esaki, Y Sato, T Murata, Y Nishiyama, D Watanabe, H Kimura
    Am J Cancer Res, 2017-08-01;7(8):1693-1703.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Selection
  12. PAX7 expression defines germline stem cells in the adult testis.
    Authors: Aloisio G, Nakada Y, Saatcioglu H, Pena C, Baker M, Tarnawa E, Mukherjee J, Manjunath H, Bugde A, Sengupta A, Amatruda J, Cuevas I, Hamra F, Castrillon D
    J Clin Invest, 2014-08-18;124(9):3929-44.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  13. Oncolytic viral therapy with a combination of HF10, a herpes simplex virus type 1 variant and granulocyte-macrophage colony-stimulating factor for murine ovarian cancer.
    Authors: Goshima F, Esaki S, Luo C, Kamakura M, Kimura H, Nishiyama Y
    Int J Cancer, 2013-12-02;134(12):2865-77.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Non-thermal Nanoelectroablation of UV-induced Murine Melanomas Stimulates an Immune Response
    Authors: Richard Nuccitelli, Kevin Tran, Kaying Lui, Joanne Huynh, Brian Athos, Mark Kreis et al.
    Pigment Cell & Melanoma Research
  15. Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.
    J Neuroinflammation, 2012-08-10;9(0):193.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. CXCR3-dependent recruitment of antigen-specific T lymphocytes to the liver during murine cytomegalovirus infection.
    Authors: Hokeness KL, Deweerd ES, Munks MW, Lewis CA, Gladue RP, Salazar-Mather TP
    J. Virol., 2006-11-15;81(3):1241-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  17. Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide.
    Authors: Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO
    J. Invest. Dermatol., 2006-04-20;126(8):1792-803.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Reviews for Mouse CD4 Antibody

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Mouse CD4 Antibody
By Anonymous on 08/24/2021
Application: Protein:Protein interaction Sample Tested: Recombinant protein Species: Mouse

Binds to murine CD4-his. Immobilize CD4-his on Penta-his sensors, and flow 3 conc of Anti-murine CD4 (100, 50, 25nM). Specific binding of Anti-murine CD4 to murine CD4, no binding to human CD4.

Mouse CD4 Antibody
By Anonymous on 05/30/2017
Application: Flow Sample Tested: peritoneal granulocytes Species: Mouse