Mouse CD300a/LMIR1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1186A
Detection of CD300a/LMIR1 in T1165 Mouse Cell Line by Flow Cytometry.
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Mouse CD300a/LMIR1 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD300a/LMIR1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LMIR1, 2, 3, 4, 5, 6, recombinant mouse LMIR3, 4, or 5 is observed.
Source
Monoclonal Rat IgG2A Clone # 172224
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD300a/LMIR1
Leu28-Arg183
Accession # BAC80268
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD300a/LMIR1 antibody in T1165 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CD300a/LMIR1 in T1165 Mouse Cell Line by Flow Cytometry. T1165 mouse plasmacytoma cell line was stained with Rat Anti-Mouse CD300a/LMIR1 APC-conjugated Monoclonal Antibody (Catalog # FAB1186A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD300a/LMIR1

CD300a, also known as LMIR1, CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse CD300a consists of a 158 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 112 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2, 3). Within the ECD, mouse CD300a shares 40% and 66% aa sequence identity with human and rat CD300a, respectively. Alternate splicing generates an additional mouse CD300a isoform with a 4 aa deletion following the Ig-like domain (3). In mouse, CD300a is expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells (2‑4). Antibody cross‑linking of CD300a induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Cross‑linking of CD300a to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (4, 8‑10). CD300a cross‑linking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 7, 9).

References
  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.
  3. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
  4. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.
  5. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.
  6. Munitz, A. et al. (2006) Blood 107:1996.
  7. Bachelet, I. et al. (2005) J. Immunol. 175:7989.
  8. Bachelet, I. et al. (2008) J. Immunol. 180:6064.
  9. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.
  10. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.
Entrez Gene IDs
11314 (Human); 217303 (Mouse)
Alternate Names
CD300a antigenCMRF35H9; CD300a molecule; CD300a; CLM8; CLM-8; CMRF35H leukocyte immunoglobulin-like receptor; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF-35-H9CD300 antigen-like family member A; CMRF35HIRp60; CMRF35-like molecule 8; IGSF12; IGSF12NK inhibitory receptor; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; leukocyte membrane antigen; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4

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