Mouse CD200 Biotinylated Antibody

Catalog #: BAF3355 Datasheet / COA / SDS

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BAF3355

All CD200 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD200 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD200 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human CD200 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD200
Gln31-Gly232
Accession # O54901

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD)

Flow Cytometry

2.5 µg/10⁶ cells

Mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD200

CD200, also known as OX-2, is a 45 kDa type I transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The mouse CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-likeV-type and one Ig-like C2-type domain (3). Splice variants of CD200 have been described in human but not in mouse. Within the ECD, mouse CD200 shares 76% and 94% aa sequence identity with human and rat CD200, respectively.

References

Entrez Gene IDs

4345 (Human); 17470 (Mouse); 102146004 (Cynomolgus Monkey)

Alternate Names

antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2