Mouse CD151 Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4609T-100UG
Detection of CD151 in bEnd.3 Mouse Cell Line by Flow Cytometry
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Mouse CD151 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD151. Stains mouse CD151 transfectants but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 455807
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with mouse CD151
Met1-Tyr253
Accession # BAA22447
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD151 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry View Larger

Detection of CD151 in bEnd.3 Mouse Cell Line by Flow Cytometry bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse CD151 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB4609T, filled histogram) or isotype control antibody (Catalog # IC006T, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD151

CD151 is a 29 kDa palmitoylated glycoprotein in the tetraspanin superfamily and is expressed by endothelial cells, epithelial cells, megakaryocytes, and platelets. It associates with other tetraspanin proteins and with some integrins including alpha 3/ beta 1, alpha 6/ beta 1, alpha 6/ beta 4, and alpha 7/ beta 1. CD151 modulates cellular adhesion, cell migration, and platelet activation. Mouse CD151 shares 94%-96% aa sequence identity with human and rat CD151.

Entrez Gene IDs
977 (Human); 12476 (Mouse); 64315 (Rat)
Alternate Names
CD151 antigen (Raph blood group); CD151 antigenplatelet surface glycoprotein gp27; CD151 molecule (Raph blood group); CD151; GP27; Membrane glycoprotein SFA-1; MER2; PETA3; PETA-3; PETA-3SFA-1; platelet-endothelial cell tetraspan antigen 3; Platelet-endothelial tetraspan antigen 3; RAPH; SFA1; SFA-1; tetraspanin-24; TSPAN24; tspan-24; TSPAN24hemidesmosomal tetraspanin CD151

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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