Mouse CCR7 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3477A-025
FAB3477A-100
Detection of CCR7 in Mouse CD4+Splenocytes by Flow Cytometry.
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Product Details
Citations (1)
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Mouse CCR7 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR7.
Source
Monoclonal Rat IgG2A Clone # 4B12
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
RBC-2H3 cells expressing mouse CCR7
Accession # NP_031745
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR7 antibody in Mouse CD4<SUP>+</SUP>Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR7 in Mouse CD4+Splenocytes by Flow Cytometry. Mouse CD4+splenocytes were stained with Rat Anti-Mouse CCR7 APC-conjugated Monoclonal Antibody (Catalog # FAB3477A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Mouse CCR7 by Flow Cytometry View Larger

Detection of Mouse CCR7 by Flow Cytometry B6 and CD69−/− CD4 T cells differ in the expression of naïve and memory surface cell markers.Cells were isolated from the spleen (spl), mesenteric lymph nodes (MLN), small intestinal lamina propria (siLP), colonic lamina propria (cLP) and blood (bld) of non-treated B6 and CD69−/− mice and analyzed by flow cytometry. The surface expression of CD44 (A), CD103 (B), CD62L (C), CCR-7 (D), CCR-9 (E) and alpha 4 beta 7 integrin (F) by CD4 T cells were analysed. Graphs represent mean (± SEM) of CD4 T cell fraction expressing the indicated molecule for four mice per each strain per tissue. Black bars represent data for B6 and white bars for CD69−/− cells. *p≤0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23776480), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR7

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Mouse CCR7 shares 87% amino acid sequence identity with human CCR7.

Entrez Gene IDs
1236 (Human); 12775 (Mouse); 287673 (Rat)
Alternate Names
BLR2; BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7; CMKBR7chemokine (C-C) receptor 7; EBI1; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

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Citation for Mouse CCR7 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The Early Activation Marker CD69 Regulates the Expression of Chemokines and CD4 T Cell Accumulation in Intestine
    Authors: Katarina Radulovic, Valerio Rossini, Calin Manta, Karlheinz Holzmann, Hans A. Kestler, Jan Hendrik Niess
    PLoS ONE

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