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Mouse CCL1/I-309/TCA-3 Antibody

Catalog #: AF845
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF845
AF845-SP
Chemotaxis Induced by CCL1/ITCA‑3 and Neutralization by Mouse CCL1/ITCA‑3 Antibody.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL1/I-309/TCA-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CCL1/I-309/TCA-3
Lys24-Cys92
Accession # P10146
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL1/I‑309/TCA‑3 (Catalog # 845-TC)
Neutralization
Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR8. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 0.01 µg/mL Recombinant Mouse CCL1/I‑309/TCA‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL1/ITCA‑3 and Neutralization by Mouse CCL1/ITCA‑3 Antibody. View Larger

Chemotaxis Induced by CCL1/ITCA‑3 and Neutralization by Mouse CCL1/ITCA‑3 Antibody. Recombinant Mouse CCL1/ TCA-3 (Catalog # 845-TC) chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/ TCA-3 (0.01 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Mouse CCL1/TCA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF845). The ND50 is typically 0.3-1.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL1/I-309/TCA-3

Mouse TCA-3 is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

TCA-3 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA‑3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta, and JE. TCA-3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T cells.

References
  1. Goya, I. et al. (1998) J. Immunol. 160:1975.
  2. Burd, P. et al. (1987) J. Immunol. 139:3126.
Entrez Gene IDs
6346 (Human); 20290 (Mouse)
Alternate Names
C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3

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Citations for Mouse CCL1/I-309/TCA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CCR8(+)FOXp3(+) Treg cells as master drivers of immune regulation
    Authors: Y Barsheshet, G Wildbaum, E Levy, A Vitenshtei, C Akinseye, J Griggs, SA Lira, N Karin
    Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL-12 produced by dendritic cells augments CD8+ T cell activation through the production of the chemokines CCL1 and CCL17.
    Authors: Henry CJ, Ornelles DA, Mitchell LM, Brzoza-Lewis KL, Hiltbold EM
    J. Immunol., 2008-12-15;181(12):8576-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Protumoral TSP50 Regulates Macrophage Activities and Polarization via Production of TNF-alpha and IL-1 beta, and Activation of the NF-kappa B Signaling Pathway
    Authors: Cheng Yang, Dong-Mei Zhang, Zhen-Bo Song, Ya-Qin Hou, Yong-Li Bao, Lu-Guo Sun et al.
    PLOS ONE

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