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Mouse Cathepsin E Antibody

Catalog #: MAB1130
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1130
MAB1130-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cathepsin E Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cathepsin E in direct ELISAs and Western blots. It recognizes both the pro and active forms of recombinant mouse (rm) Cathepsin E. In Western blots, no cross-reactivity with rmCathepsin B, C, H, L, or recombinant human Cathepsin O, S, or V is observed.
Source
Monoclonal Rat IgG2A Clone # 186513
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin E
Gln19-Pro397
Accession # P70269
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Cathepsin E (Catalog # 1130-AS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin E

Cathepsin E is an intracellular aspartic protease of the pepsin family (1, 2). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsins D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (3). Both enzymes are efficient in cleaving the Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with the wild-type APP (4). Mouse Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1-18), a propeptide (residues 19-59), and a mature chain (residues 60-397) (1).

References
  1. Tatnell, P.J. et al. (1997) FEBS Lett. 408:62.
  2. Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.), p. 33,  Academic Press, San Diego.
  3. Tsukuba, T. et al. (2000) Mol. Cells 10:601.
  4. Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.
Entrez Gene IDs
1510 (Human); 13034 (Mouse)
Alternate Names
CATE; Cathepsin E; CTSE; EC 3.4.23; EC 3.4.23.34; erythrocyte membrane aspartic proteinase; slow-moving proteinase

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Citation for Mouse Cathepsin E Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. An essential role for the antiviral endoribonuclease, RNase-L, in antibacterial immunity.
    Authors: Li XL, Ezelle HJ, Kang TJ, Zhang L, Shirey KA, Harro J, Hasday JD, Mohapatra SK, Crasta OR, Vogel SN, Cross AS, Hassel BA
    Proc. Natl. Acad. Sci. U.S.A., 2008-12-15;105(52):20816-21.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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