Mouse c-Rel Biotinylated Antibody

Name: Mouse c-Rel Biotinylated Antibody
Brand: R&D Systems
SKU: BAM2699
Rating: 5 (1 reviews)

Catalog #: BAM2699
1 Citation 1 Review Datasheet / COA / SDS

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BAM2699

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse c-Rel Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse c-Rel.

Source

Monoclonal Rat IgG_2A Clone # 290512

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse c-Rel
Met1-Ile588
Accession # P15307

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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c‑Rel in Mouse Splenocytes. c‑Rel was detected in immersion fixed mouse splenocytes using 10 µg/mL Mouse c‑Rel Biotinylated Monoclonal Antibody (Catalog # BAM2699) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: c-Rel

c-Rel is a member of the NF kappa B/Rel family of transcription factors. NF kappa B/Rel dimers containing c-Rel regulate expression of genes involved in lymphoid cell development, proliferation, and survival.

Long Name

Cellular Repressor of E1A-stimulated Genes

Entrez Gene IDs

5966 (Human); 19696 (Mouse)

Alternate Names

C-Rel proto-oncogene protein; cRel; c-Rel; I-Rel; oncogene REL, avian reticuloendotheliosis; proto-oncogene c-Rel; REL; v-rel avian reticuloendotheliosis viral oncogene homolog; v-rel reticuloendotheliosis viral oncogene homolog (avian)

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse c-Rel Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Overexpression of the Nuclear Factor-{kappa}B subunit c-Rel protects against human islet cell death in vitro.
Authors: Mokhtari D, Barbu A, Mehmeti I, Vercamer C, Welsh N
Am. J. Physiol. Endocrinol. Metab., 2009-08-25;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P