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Mouse B7-H4 Biotinylated Antibody

Catalog #: BAF2154
2 Citations Datasheet / COA / SDS
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BAF2154
Detection of B7-H4 in HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP by Flow Cytometry.
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Citations (2)
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Mouse B7-H4 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse B7-H4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) B7‑1, rmB7‑2, rmB7‑H1, rmB7‑H2, rmB7‑H3, and rmPD‑L2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7-H4
Phe29-Pro258
Accession # Q7TSP5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse B7-H4 (Catalog # 2154-B7)
Flow Cytometry
0.25 µg/106 cells
HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of B7-H4 antibody in HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP antibody by Flow Cytometry. View Larger

Detection of B7-H4 in HEK293 Human Cell Line Transfected with Mouse B7-H4 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H4 or (B) irrelevant protein and eGFP was stained with Goat Anti-Human/Mouse B7-H4 Biotinylated Polyclonal Antibody (Catalog # BAF2154) followed by APC-conjugated Streptavidin (F0050). Quadrant markers were set based on Goat IgG control antibody staining (Catalog # BAF108). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H4

B7-H4, also known as B7x and B7S1, is a member of the B7 family of immune co-stimulatory proteins (1). Mature B7-H4 is a 50 kDa - 80 kDa glycosylated molecule with a 28 kDa protein core (2). Partial sensitivity to cleavage by PI-PLC suggests a possible GPI linkage of mouse B7-H4 to the cell membrane (3). The 230 amino acid (aa) extracellular region of B7-H4 contains one Ig-like V-set domain and one Ig-like C2-set domain which is followed by a hydrophobic C-terminal region (3 - 5). Within the ECD, mouse B7-H4 shares 90% and 99% aa sequence identity with human and rat B7-H4, respectively. It shares 21% - 29% aa sequence identity with B7-1, B7-2, B7-H1, B7-H2, B7-H3, and PD-L2. B7-H4 expression is induced on mitogen- or LPS-activated B cells, T cells, dendritic cells, monocytes, and macrophages (3, 4) and blocked by GM-CSF or IL-4 (6). It is also expressed on various normal epithelia and upregulated in several carcinomas and renal tubule epithelial cell lesions (2, 6 - 10). B7-H4 is expressed on the surface of macrophages but intracellularly in ovarian and breast cancer cells (2, 6 - 7). It is found in the serum and ascites fluid of cancer patients (11). B7-H4 binds an unidentified ligand on activated T cells which is distinct from BTLA, CD28, CTLA4, ICOS, and PD-1 (3 - 5, 12). Exposure to B7-H4 inhibits antigen-dependent induction of T cell proliferation and activation (3 - 5). Alternatively, B7-H4 expressing renal tubular epithelial cells promote T cell activation (8). Regulatory T cells, IL-6, and IL-10 induce B7-H4 expression on antigen presenting cells which fosters tumor growth by dampening the anti-tumor immune response (6, 13). B7-H4 also promotes the malignant transformation of epithelial cells by protecting them from apoptosis (2). Despite evidence for the involvement of B7-H4 in immune regulation, mice deficient in its expression do not show significant immune deficiencies, suggesting compensation by other molecules in vivo (14).

References
  1. Greenwald, R.J. et al. (2005) Annu. Rev. Immunol. 23:515.
  2. Salceda, S. et al. (2005) Exp. Cell Res. 306:128.
  3. Prasad, V.R. et al. (2003) Immunity 18:863.
  4. Sica, G.L. et al. (2003) Immunity 18:849.
  5. Zang, X. et al. (2003) Proc. Natl. Acad. Sci. 100:10388.
  6. Kryczek, I. et al. (2006) J. Exp. Med. 203:871.
  7. Tringler, B. et al. (2005) Clin. Cancer Res. 11:1842.
  8. Chen, Y. et al. (2006) Kidney Int. 70:2092.
  9. Choi, I.-H. et al. (2003) J. Immunol. 171:4650.
  10. Krambeck, A.E. et al. (2006) Proc. Natl. Acad. Sci. 103:10391.
  11. Simon, I. et al. (2006) Cancer Res. 66:1570.
  12. Sedy, J.R. et al. (2005) Nat. Immunol. 6:90.
  13. Kryczek, I. et al. (2006) J. Immunol. 177:40.
  14. Suh, W.-K. et al. (2006) Mol. Cell. Biol. 26:6403.
Long Name
B7 Homolog 4
Entrez Gene IDs
79679 (Human); 242122 (Mouse); 295322 (Rat)
Alternate Names
B7h.5; B7H4; B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1; B7S1VCTN1; B7x; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; Vtcn1

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Citations for Mouse B7-H4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. B7x in the periphery abrogates pancreas-specific damage mediated by self-reactive CD8 T cells.
    Authors: Lee J, Scandiuzzi L, Ray A, Wei J, Hofmeyer K, Abadi Y, Loke P, Lin J, Yuan J, Serreze D, Allison J, Zang X
    J Immunol, 2012-09-12;189(8):4165-74.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  2. Tissue-expressed B7x affects the immune response to and outcome of lethal pulmonary infection.
    Authors: Hofmeyer, Kimberly, Scandiuzzi, Lisa, Ghosh, Kaya, Pirofski, Liise-An, Zang, Xingxing
    J Immunol, 2012-08-01;189(6):3054-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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