Mouse alpha-Fetoprotein/AFP Biotinylated Antibody

Catalog #: BAF5369 Datasheet / COA / SDS

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BAF5369

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Mouse alpha-Fetoprotein/AFP Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse alpha ‑Fetoprotein/AFP in Western blots. In Western blots, approximately 10% cross-reactivity with human alpha -Fetoprotein is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse alpha ‑Fetoprotein/AFP
Lys19-Val605
Accession # P02772

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse alpha ‑Fetoprotein/AFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Fetoprotein/AFP

AFP ( alpha -Fetoprotein)is a 69-73 kDa member of the ALB/AFP/VDB family of proteins. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature mouse AFP is 587 amino acids (aa) in length. It contains three albumin domains (aa 20-201, 208-393 and 400-591), plus 15 intrachain disulfide bonds. Rat AFP contains an alternate start site at Met287 that may generate an intracellular 37 kDa form. Mouse AFP also shows an analogous site at Met281 that may produce an equivalent isoform. Over aa 19-605, mouse AFP shares 83% and 66% aa identity with rat and human AFP, respectively.

Entrez Gene IDs

174 (Human); 11576 (Mouse); 24177 (Rat)

Alternate Names

AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP