Mouse alpha-Fetoprotein/AFP Antibody

Detection of Mouse alpha -Fetoprotein/AFP by Western Blot. Western blot shows lysates of mouse plasma and rat placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse a-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Mouse alpha-Fetoprotein/AFP by Western Blot Extensive hepatocyte damage by hepatocyte-specific activation of FOXO3 associated with upregulation of HCC-concomitant factors. a More inflammation can be observed in livers from 9-week-old FOXO3CAHep transgenic mice than in control animals. b Expression of Trp53 and Myc by qPCR in livers from 9-week-old control and FOXO3CAHep transgenic mice. c Western blot analysis in livers from 9-week-old control and FOXO3CAHep mice with p53 and c-Myc and GAPDH as loading control (left panel), as well as densitometric quantification (middle and right panels). d Expression of Afp by qPCR in livers from 9-week-old control and FOXO3CAHep transgenic mice (n = 4). e Representative IHC staining for AFP (bar = 100 μm).f Western blot analysis in livers from 9-week-old control and FOXO3CAHep mice with AFP and GAPDH as loading control (upper panel), as well as densitometric quantification (lower panel). g Serum AFP levels in 9-week-old control and FOXO3CAHep mice Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31488102), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse alpha-Fetoprotein/AFP by Western Blot Enhanced proliferation and elevated expression of AFP and Vimentin in livers of DEN-injected 40-week-old FOXO3CAHep transgenic mice. a Representative IHC staining for Ki67, AFP, p53, E-Cadherin, N-Cadherin, and Vimentin in 40-week-old DEN-injected control and DEN-injected FOXO3CAHep transgenic mice (bar = 100 μm). b Scoring of Ki67-positivity (n = 4). c Western blot analysis in livers from 40-week-old DEN-injected control and DEN-injected FOXO3CAHep transgenic mice with AFP, c-Myc, p53, E-Cadherin, N-Cadherin, Vimentin and GAPDH as loading control (upper part), as well as densitometric quantification (lower part) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31488102), licensed under a CC-BY license. Not internally tested by R&D Systems.