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Mouse 5T4 Antibody

Catalog #: AF5049
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5049
AF5049-SP
Detection of 5T4 in D3 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse 5T4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse 5T4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human 5T4 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse 5T4
Ser30-Ser361
Accession # AAH58198
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse 5T4
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of 5T4 antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of 5T4 in D3 Mouse Cell Line by Flow Cytometry. Retinoic acid-treated D3 mouse embryonic stem cell line was stained with Sheep Anti-Mouse 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5049, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).

Immunocytochemistry 5T4 antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

5T4 in D3 Mouse Cell Line. 5T4 was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5049) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm/membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5T4

5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72‑80 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and differentiating embryonic stem cells and impacts cell adhesion and motility. The mouse 5T4 cDNA encodes a type I transmembrane protein precursor that is 426 amino acids (aa) in length. It contains a 332 aa extracellular region (aa 30‑361) that shows one Ser-rich region followed by seven Leu-rich repeats (aa 90‑338). Over aa 30‑361, mouse 5T4 shares 95% and 81% aa identity with rat and human 5T4, respectively.

Long Name
Oncofetal Antigen/Trophoblast Glycoprotein
Entrez Gene IDs
7162 (Human); 21983 (Mouse); 83684 (Rat); 102132149 (Cynomolgus Monkey)
Alternate Names
5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; 5T4; 5T4AG; 5T4-AG; 5T4M6P1,5T4 oncofetal antigen; M6P1; TPBG; trophoblast glycoprotein

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Citations for Mouse 5T4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Expression and distribution of trophoblast glycoprotein in the mouse retina
    Authors: Colin M. Wakeham, Gaoying Ren, Catherine W. Morgans
    Journal of Comparative Neurology
  2. Co-transmitting interneurons in the mouse olfactory bulb regulate olfactory detection and discrimination
    Authors: Lyons-Warren, AM;Tantry, EK;Moss, EH;Kochukov, MY;Belfort, BDW;Ortiz-Guzman, J;Freyberg, Z;Arenkiel, BR;
    Cell reports
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. WAIF1 is a cell-surface CTHRC1 binding protein coupling bone resorption and formation
    Authors: K Matsuoka, Y Kohara, Y Naoe, A Watanabe, M Ito, K Ikeda, S Takeshita
    J. Bone Miner. Res., 2018-05-11;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Recombinant Protein, Whole Cells
    Applications: Flow Cytometry, Immunoprecipitation, Western Blot
  4. The Stem Cell Marker Lgr5 Defines a Subset of Post-Mitotic Neurons in the Olfactory Bulb
    Authors: Y Yu, AH Moberly, JP Bhattarai, C Duan, Q Zheng, F Li, H Huang, W Olson, W Luo, T Wen, H Yu, M Ma
    J. Neurosci., 2017-08-28;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. A Subtype of Olfactory Bulb Interneurons Is Required for Odor Detection and Discrimination Behaviors
    J Neurosci, 2016-08-03;36(31):8210-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.
    Authors: Rosu-Myles M, She YM, Fair J
    PLoS ONE, 2012-06-13;7(6):e38954.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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