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Human ZNF206 Antibody

Catalog #: AF4020
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4020
AF4020-SP
ZNF206 in BG01V Human Embryonic Stem Cells.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ZNF206 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ZNF206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ZNF24 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ZNF206
Met1-Val283
Accession # Q96SZ4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ZNF206
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry ZNF206 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

ZNF206 in BG01V Human Embryonic Stem Cells. ZNF206 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human ZNF206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4020) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with tubulin (green). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZNF206

ZNF206 (zinc finger protein 206; also ZFP206) is an 80 kDa SCAN-zinc finger transcription factor that is active in embryonic stem cells and appears to positively regulate transcriptional regulators that maintain stem cell pluripotency. Human ZNF206 is 725 amino acids in length. It contains one SCAN box (aa 1‑71) that mediates oligomerization, followed by a proline-rich region (aa 104‑175) and 14 consecutive C2H2-type zinc fingers (aa 292‑719). Mouse ZNF206 apparently has multiple isoforms, none of which are well characterized. The human gene may also show alternate splicing. Human ZNF206 is 72% aa identical to mouse ZNF206.

Long Name
Zinc Finger Protein 206/Zinc Finger and SCAN Domain Containing 10
Entrez Gene IDs
84891 (Human); 332221 (Mouse); 302962 (Rat)
Alternate Names
Zfp206; ZNF206; ZSCAN10 zinc finger and SCAN domain containing 10; ZSCAN10

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Citations for Human ZNF206 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. ZSCAN10 expression corrects the genomic instability of iPSCs from aged donors
    Authors: M Skamagki, C Correia, P Yeung, T Baslan, S Beck, C Zhang, CA Ross, L Dang, Z Liu, S Giunta, TP Chang, J Wang, A Ananthanar, M Bohndorf, B Bosbach, J Adjaye, H Funabiki, J Kim, S Lowe, JJ Collins, CW Lu, H Li, R Zhao, K Kim
    Nat. Cell Biol., 2017-08-28;19(9):1037-1048.
  2. Zscan10 is dispensable for maintenance of pluripotency in mouse embryonic stem cells.
    Authors: Yamane M, Fujii S, Ohtsuka S, Niwa H
    Biochem Biophys Res Commun, 2015-11-22;468(4):826-31.

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