Human VIPR2 Antibody

Catalog #: MAB5416 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5416
MAB5416-SP

1 Image

All VIPR2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VIPR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VIP R2 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 476031

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human VIP R2 Glu24-Ile438 Accession # P41587

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

VIP R2 in Human Brain. VIP R2 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using 25 µg/mL Mouse Anti-Human VIP R2 Monoclonal Antibody (Catalog # MAB5416) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: VIPR2

Human VIP R2 (Vasoactive intestinal polypeptide receptor 2) is a 47 kDa (predicted) seven transmembrane receptor that belongs to the G protein coupled receptor 2 family of proteins. In addition to the seven transmembrane regions, the mature chain also contains in its first extracellular region a hormone receptor domain (aa 49-114), which includes three N-linked glycosylation sites. Human VIP R2 shares 85% aa identity with mouse and rat VIP R2. VIP R2 is expressed predominantly in skeletal muscle and to a lesser extent in the brain, heart, pancreas, and placenta. VIP R2 is coupled to a cAMP mediated signal transduction pathway and binds VIP and pituitary adenylate cyclase activating polypeptide (PACAP).

Long Name

Vasoactive Intestinal Peptide Receptor 2

Entrez Gene IDs

7434 (Human); 22355 (Mouse); 29555 (Rat)

Alternate Names

FLJ16511; Helodermin-preferring VIP receptor; PACAP type III receptor; PACAPR3; PACAP-R3; PACAP-R-3; Pituitary adenylate cyclase-activating polypeptide type III receptor; vasoactive intestinal peptide receptor 2; vasoactive intestinal polypeptide receptor 2; VIP R2; VIP2R; VIPR2; VIP-R-2; VPAC2; VPCAP2R