Human VHL Antibody

Catalog #: MAB114351 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB114351-100
MAB114351-SP

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Human VHL Antibody Summary

Species Reactivity

Human

Specificity

Detects human VHL in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1066409

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human VHL
Asn150-Asp213
Accession # P40337

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of Human Kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of VHL in Human Kidney. VHL was detected in immersion fixed paraffin-embedded sections of Human Kidney using Mouse Anti-Human VHL Monoclonal Antibody (Catalog # MAB114351) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in cells in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VHL

VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues. Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others. In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.

References

Kamura T., et al. (1998) Genes & Dev. doi: 10.1101/gad.12.24.3872
Okumura F., et al. (2012) Front. Oncol. doi: 10.3389/fonc.2012.00010
Xie L., et al. (2009) Sci. Signaling doi: 10.1126/scisignal.2000444
Zhang J., et al. (2018) Science doi: 10.1126/science.aap8411

Long Name

Von Hippel-Lindau Tumor Suppressor

Entrez Gene IDs

7428 (Human); 22346 (Mouse)

Alternate Names

elongin binding protein; HRCA1; Protein G7; pVHL; RCA1; VHL; VHL1; von Hippel-Lindau disease tumor suppressor; von Hippel-Lindau syndrome; von Hippel-Lindau tumor suppressor