Human VHL Antibody

Catalog #: MAB114352 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB114352-100
MAB114352-SP

Detection of VHL in Daudi Human Cell Line.

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Human VHL Antibody Summary

Species Reactivity

Human

Specificity

Detects human VHL in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1066408

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human VHL
Asn150-Asp213
Accession # P40337

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

3-25 µg/mL

Formalin fixed Daudi human Burkitt's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of VHL in Daudi Human Cell Line. VHL was detected in formalin fixed Daudi human Burkitt's lymphoma cell line using Mouse Anti-Human VHL Monoclonal Antibody (Catalog # MAB114352) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VHL

VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues. Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others. In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.

References

Kamura, T. et al. (1998) Genes & Dev. doi: 10.1101/gad.12.24.3872.
Okumura, F. et al. (2012) Front. Oncol. doi: 10.3389/fonc.2012.00010.
Xie, L. et al. (2009) Sci. Signaling doi: 10.1126/scisignal.2000444.
Zhang, J. et al. (2018) Science doi: 10.1126/science.aap8411.

Long Name

Von Hippel-Lindau Tumor Suppressor

Entrez Gene IDs

7428 (Human); 22346 (Mouse)

Alternate Names

elongin binding protein; HRCA1; Protein G7; pVHL; RCA1; VHL; VHL1; von Hippel-Lindau disease tumor suppressor; von Hippel-Lindau syndrome; von Hippel-Lindau tumor suppressor