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Human Versican Antibody

Catalog #: MAB3054
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3054
MAB3054-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Versican Antibody Summary

Species Reactivity
Human
Specificity
Detects human Versican in Western blots.
Source
Monoclonal Rat IgG1 Clone # 255915
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Versican isoform V0
Gly1344-Asp1554
Accession # P13611
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Versican
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Versican, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Versican

Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa 21-346), two large, alternately spliced GAG attachment domains (GAG alpha ; aa 348-1335; GAG beta aa 1336-3088) and a C-terminal selectin-like region (G3; aa 3089-3396). Over aa 1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.

Entrez Gene IDs
1462 (Human); 13003 (Mouse)
Alternate Names
Chondroitin sulfate proteoglycan 2WGN; Chondroitin sulfate proteoglycan core protein 2; CSPG2; CSPG2DKFZp686K06110; ERVR; GHAP; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; PG-M; PG-MWGN1; VCAN; versican core protein; versican proteoglycan; Versican

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Citations for Human Versican Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Mechanisms of Hypoxic Up-Regulation of Versican Gene Expression in Macrophages.
    Authors: Sotoodehnejadnematalahi F, Staples K, Chrysanthou E, Pearson H, Ziegler-Heitbrock L, Burke B
    PLoS ONE, 2015-06-09;10(6):e0125799.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Glomerular endothelial glycocalyx constitutes a barrier to protein permeability.
    Authors: Singh A, Satchell SC, Neal CR
    J. Am. Soc. Nephrol., 2007-10-17;18(11):2885-93.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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