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Human VE-Cadherin Antibody

Catalog #: MAB9381
28 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9381
MAB9381-SP
Detection of Human VE‑Cadherin by Western Blot.
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Product Details
Citations (28)
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Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VE-Cadherin Antibody Summary

Species Reactivity
Human
Specificity
Detects human VE-Cadherin in Western blots. In Western blots, 25% cross-reactivity with recombinant mouse VE‑Cadherin and
no cross‑reactivity with recombinant human (rh) Cadherin-17 or rhP-Cadherin is observed.
Source
Monoclonal Mouse IgG2B Clone # 123413
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
Asp48-Gln593
Accession # P33151
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
HUVEC human umbilical vein endothelial cells stained in buffer containing Ca2+ and Mg2+
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
0.5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human VE-Cadherin antibody by Western Blot. View Larger

Detection of Human VE‑Cadherin by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VE-Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry VE-Cadherin antibody in HUVEC Cells by Immunocytochemistry (ICC). View Larger

VE‑Cadherin in HUVEC Cells. VE-Cadherin was detected in immersion fixed HUVEC cells using Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry Detection of Human VE-Cadherin by Flow Cytometry View Larger

Detection of Human VE-Cadherin by Flow Cytometry Immortalized HAECs retain phenotypic and functional characteristics of primary cells. (A) (Top) Immortalized HAEC confluent monolayers stained with CD31-AF488 antibody (green) and DAPI (blue). (Bottom) Flow cytometry histogram plots of primary or immortalized HAECs labeled with CD31-AF488 antibody. Secondary antibody only (dashed line) and nonstained cells (dotted line) were used as negative controls. (B) (Top) Immortalized HAEC confluent monolayers stained with VE-cadherin-AF488 (green) antibody, Acti-Stain 555 phalloidin (red), and DAPI (blue). (Bottom) Flow cytometry histogram plots of immortalized HAECs labeled with VE-cadherin-AF488 and ZO-1-AF647 antibodies. Secondary antibody only (dashed line) was used as a negative control. (C) Tube forming assay of primary (top) and immortalized (bottom) HAECs at 0 h and 8 h after seeding in Matrigel-coated wells. (D) Acetylated low-density lipoprotein (Ac-LDL) uptake assay of primary HAECs (top), immortalized HAECs (middle), and fibroblasts (negative control; bottom) treated with fluorescently labeled Ac-LDL (red) for 2.5 h and stained with DAPI (blue). (E) IL-8 detection in culture supernatants 24 h after treatment of primary or immortalized HAECs with increasing concentrations of TNF-alpha, IFN-gamma, or IL-6. (F) IL-8 detection in culture supernatants 24 h after treatment of immortalized HAECs with increasing concentrations of IL-2 or IL-1. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29229737), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VE-Cadherin

Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-cadherin proteins share approximately 74% homology.

References
  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.
  6. Carmeliet, P. et al. (1999) Cell 98:147.
  7. Gory-Faure, S. et al. (1999) Development 126:2093.
Long Name
Vascular Endothelium Cadherin
Entrez Gene IDs
1003 (Human); 12562 (Mouse)
Alternate Names
7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin

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Citations for Human VE-Cadherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. The Multi-Kinase Inhibitor RepSox Enforces Barrier Function in the Face of Both VEGF and Cytokines
    Authors: Lietuvninkas, L;Baccouche, B;Kazlauskas, A;
    Biomedicines
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. PIEZO1 and PECAM1 interact at cell-cell junctions and partner in endothelial force sensing
    Authors: Eulashini Chuntharpursat-Bon, Oleksandr V. Povstyan, Melanie J. Ludlow, David J. Carrier, Marjolaine Debant, Jian Shi et al.
    Communications Biology
  3. Mesenchymal stem cells support human vascular endothelial cells to form vascular sprouts in human platelet lysate-based matrices
    Authors: S Summer, E Rossmanith, M Pasztorek, C Fiedler, M Gröger, S Rauscher, V Weber, MB Fischer
    PLoS ONE, 2022-12-15;17(12):e0278895.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Hypoxia induces purinergic receptor signaling to disrupt endothelial barrier function
    Authors: Somasundaram Raghavan, Masuma Akter Brishti, Daniel Mohr Collier, M. Dennis Leo
    Frontiers in Physiology
  5. FSAP Protects against Histone-Mediated Increase in Endothelial Permeability In Vitro
    Authors: XY Cui, B Stavik, B Thiede, PM Sandset, SM Kanse
    International Journal of Molecular Sciences, 2022-11-08;23(22):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Endothelial deletion of TBK1 contributes to BRB dysfunction via CXCR4 phosphorylation suppression
    Authors: Bowen Zhao, Yueqi Ni, Hong Zhang, Yin Zhao, Lu Li
    Cell Death Discovery
  7. A combined human gastruloid model of cardiogenesis and neurogenesis
    Authors: Olmsted ZT, Paluh JL.
    iScience
  8. Spatiotemporally controlled, aptamers-mediated growth factor release locally manipulates microvasculature formation within engineered tissues
    Authors: Deepti Rana, Ajoy Kandar, Nasim Salehi-Nik, Ilyas Inci, Bart Koopman, Jeroen Rouwkema
    Bioactive Materials
  9. The LINC complex is required for endothelial cell adhesion and adaptation to shear stress and cyclic stretch
    Authors: Kevin B. Denis, Jolene I. Cabe, Brooke E. Danielsson, Katie V. Tieu, Carl R. Mayer, Daniel E. Conway
    Molecular Biology of the Cell
  10. Differential Responses of Transplanted Stem Cells to Diseased Environment Unveiled by a Molecular NIR-II Cell Tracker
    Authors: Hao Chen, Huaxiao Yang, Chen Zhang, Si Chen, Xin Zhao, Mark Zhu et al.
    Research (Wash D C)
  11. Endothelial C3a receptor mediates vascular inflammation and blood-brain barrier permeability during aging
    Authors: Nicholas E. Propson, Ethan R. Roy, Alexandra Litvinchuk, Jörg Köhl, Hui Zheng
    Journal of Clinical Investigation
  12. Lowering the increased intracellular pH of human-induced pluripotent stem cell-derived endothelial cells induces formation of mature Weibel-Palade bodies
    Authors: Gesa L. Tiemeier, Rozemarijn de Koning, Gangqi Wang, Sarantos Kostidis, Rosalie G. J. Rietjens, Wendy M. P. J. Sol et al.
    Stem Cells Translational Medicine
  13. Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture
    Authors: R Daum, D Visser, C Wild, L Kutuzova, M Schneider, G Lorenz, M Weiss, S Hinderer, UA Stock, M Seifert, K Schenke-La
    Cells, 2020-03-23;9(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Therapeutic Myeloperoxidase Inhibition Attenuates Neutrophil Activation, ANCA-Mediated Endothelial Damage, and Crescentic GN
    Authors: Marilina Antonelou, Erik Michaëlsson, Rhys D.R. Evans, Chun Jing Wang, Scott R. Henderson, Lucy S.K. Walker et al.
    Journal of the American Society of Nephrology
  15. Adipose tissue–derived stromal cells’ conditioned medium modulates endothelial‐mesenchymal transition induced by IL‐1 beta /TGF‐ beta 2 but does not restore endothelial function
    Authors: Tácia Tavares Aquinas Liguori, Gabriel Romero Liguori, Luiz Felipe Pinho Moreira, Martin Conrad Harmsen
    Cell Proliferation
  16. Complement activation on neutrophils initiates endothelial adhesion and extravasation
    Authors: Antonina Akk, Luke E. Springer, Lihua Yang, Samantha Hamilton-Burdess, John D. Lambris, Huimin Yan et al.
    Molecular Immunology
  17. Heparanase-2 protects from LPS-mediated endothelial injury by inhibiting TLR4 signalling
    Authors: Y Kiyan, S Tkachuk, K Kurselis, N Shushakova, K Stahl, D Dawodu, R Kiyan, B Chichkov, H Haller
    Sci Rep, 2019-09-19;9(1):13591.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Human Fibrinogen for Maintenance and Differentiation of Induced Pluripotent Stem Cells in Two Dimensions and Three Dimensions
    Authors: Jarel K. Gandhi, Travis Knudsen, Matthew Hill, Bhaskar Roy, Lori Bachman, Cynthia Pfannkoch-Andrews et al.
    Stem Cells Translational Medicine
  19. Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis
    Authors: T Neri, E Hiriart, PP van Vliet, E Faure, RA Norris, B Farhat, B Jagla, J Lefrancois, Y Sugi, T Moore-Morr, S Zaffran, RS Faustino, AC Zambon, JP Desvignes, D Salgado, RA Levine, JL de la Pomp, A Terzic, SM Evans, R Markwald, M Pucéat
    Nat Commun, 2019-04-26;10(1):1929.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis
    Authors: BL Ecker, A Kaur, SM Douglass, MR Webster, FV Almeida, GE Marino, AJ Sinnamon, MG Neuwirth, GM Alicea, A Ndoye, M Fane, X Xu, MS Sim, GB Deutsch, MB Faries, GC Karakousis, AT Weeraratna
    Cancer Discov, 2018-10-02;9(1):82-95.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC
  21. Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages
    Authors: J Li, F Xing, F Chen, L He, KF So, Y Liu, J Xiao
    Cell Transplant, 2018-06-13;0(0):9636897187803.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function
    Authors: K Kulhankova, KJ Kinney, JM Stach, FA Gourronc, IM Grumbach, AJ Klingelhut, W Salgado-Pa
    Infect. Immun., 2018-02-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  23. A Three-Dimensional Cell Culture System To Model RNA Virus Infections at the Blood-Brain Barrier
    Authors: JC Bramley, CG Drummond, NJ Lennemann, CA Good, KS Kim, CB Coyne
    mSphere, 2017-06-21;2(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
    Authors: Gabriela Odent Grig
    J Tissue Eng Regen Med, 2016-12-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Targeting Syndecan-1, a molecule implicated in the process of vasculogenic mimicry, enhances the therapeutic efficacy of the L19-IL2 immunocytokine in human melanoma xenografts
    Authors: Paola Orecchia, Romana Conte, Enrica Balza, Gabriella Pietra, Maria Cristina Mingari, Barbara Carnemolla
    Oncotarget
  26. Abluminal Stimulation of Sphingosine 1-Phosphate Receptors 1 and 3 Promotes and Stabilizes Endothelial Sprout Formation
    Authors: Anusuya Das, Steven M. Lenz, Anthony O. Awojoodu, Edward A. Botchwey
    Tissue Engineering Part A
  27. Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
    Authors: Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM
    Cell Stem Cell, 2008-07-03;3(1):55-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  28. Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
    Authors: Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P
    Am. J. Physiol. Heart Circ. Physiol., 2007-03-23;293(1):H610-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

  1. Why does the staining protocol with this Cadherin antibody use buffers containing Ca2+ and Mg2+?

    • The staining protocol with this and other Cadherin antibodies uses buffer containing Ca2+ and Mg2+ because Cadherin function is Calcium-dependent. 

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Reviews for Human VE-Cadherin Antibody

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Human VE-Cadherin Antibody
By Anonymous on 04/05/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human
Human VE-Cadherin Antibody
By Anonymous on 09/10/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Human Coronary Artery Endothelial Cells Species: Human