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Human VCAM-1/CD106 Antibody

Catalog #: AF809
10 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF809
AF809-SP
Detection of Human VCAM‑1/CD106 by Western Blot.
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VCAM-1/CD106 Antibody Summary

Species Reactivity
Human
Specificity
Detects VCAM-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse VCAM-1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line (CHO)-derived recombinant human VCAM-1
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
HUVEC human umbilical vein endothelial cells
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human VCAM-1/CD106 (Catalog # ADP5, 2.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human VCAM-1/CD106 antibody by Western Blot. View Larger

Detection of Human VCAM‑1/CD106 by Western Blot. Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human VCAM-1/ CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF809) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for VCAM-1/CD106 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human VCAM‑1/CD106 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 10 ng/ml Recombinant Human TNF‑ alpha (210-TA) for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 132 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF809) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha, and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity.

During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.

ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Long Name
Vascular Cell Adhesion Molecule 1
Entrez Gene IDs
7412 (Human); 22329 (Mouse)
Alternate Names
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

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Citations for Human VCAM-1/CD106 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Integrin signaling is critical for myeloid-mediated support of T-cell acute lymphoblastic leukemia
    Authors: Lyu, A;Humphrey, RS;Nam, SH;Durham, TA;Hu, Z;Arasappan, D;Horton, TM;Ehrlich, LIR;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay, Flow Cytometry
  2. Mesenchymal stem cell aggregation mediated by integrin alpha 4/VCAM-1 after intrathecal transplantation in MCAO rats
    Authors: Ye Ran, Yankai Dong, Yuejiao Li, Jundong Xie, Shubin Zeng, Chuanlei Liang et al.
    Stem Cell Research & Therapy
  3. Human CD4+ T cell subsets differ in their abilities to cross endothelial and epithelial brain barriers in vitro
    Authors: Hideaki Nishihara, Sasha Soldati, Adrien Mossu, Maria Rosito, Henriette Rudolph, William A. Muller et al.
    Fluids and Barriers of the CNS
  4. Mesenchymal Stem Cells Transmigrate Between and Directly Through Tumor Necrosis Factor-alpha-Activated Endothelial Cells Via Both Leukocyte-Like and Novel Mechanisms.
    Authors: Teo G, Ankrum J, Martinelli R, Boetto S, Simms K, Sciuto T, Dvorak A, Karp J, Carman C
    Stem Cells, 2012-11-01;30(11):2472-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  5. N-octanoyl-dopamine is an agonist at the capsaicin receptor TRPV1 and mitigates is chemia-induced acute kidney injury in rat.
    Authors: Tsagogiorgas C, Wedel J, Hottenrott M, Schneider M, Binzen U, Greffrath W, Treede R, Theisinger B, Theisinger S, Waldherr R, Kramer B, Thiel M, Schnuelle P, Yard B, Hoeger S
    PLoS ONE, 2012-08-20;7(8):e43525.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012-02-17;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  7. The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
    Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
    Arthritis Rheum., 2007-06-01;56(6):1817-26.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  8. Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
    Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
    Blood, 2005-11-29;107(6):2252-61.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  9. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
    Authors: Carman CV, Springer TA
    J. Cell Biol., 2004-10-25;167(2):377-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses
    Authors: Rivera-Serrano EE, Gonzalez-Lopez O, Das A, Lemon SM.
    Elife

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Reviews for Human VCAM-1/CD106 Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Human VCAM-1/CD106 Antibody
By Anonymous on 07/23/2019
Application: ELISA Sample Tested: EDTA Plasma Species: Human
ELISA Human VCAM-1/CD106 Antibody AF809
Human VCAM‑1/CD106 Antibody
By Anonymous on 03/23/2018
Application: WB Sample Tested: Purified protein Species: Mouse
Human VCAM‑1/CD106 Antibody
By Anonymous on 11/03/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

The VCAM antibody (AF809) was used as the capture antibody in a competitive ELISA assay. It was absorbed onto an ELISA plate. The competitor was recombinant VCAM (809-VR) that had been biotinylated. The calibrator curve also used recombinant VCAM (809-VR). Normal human serum and plasma samples were all quantifiable.