Human VASA Biotinylated Antibody

Catalog #: BAF2030 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF2030

All VASA Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VASA Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human VASA in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human VASA
Met1-Tyr145
Accession # Q9NQI0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human VASA

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VASA

The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is therefore a highly specific marker of germ cells (1-3).

References

Zeeman, A.M. et al. (2002) Lab Invest. 82:159.
Raz, E. (2000) Genome Biol. 1:REVIEWS1017.
Castrillon, D. et al. (2001) Proc. Natl. Acad. Sci. USA 97:9585.

Long Name

DEAD [Asp-Glu-Ala-Asp] Box Polypeptide 41

Entrez Gene IDs

54514 (Human); 13206 (Mouse); 310090 (Rat)

Alternate Names

DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD box protein 4; EC 3.6.1; EC 3.6.4.13; MGC111074; probable ATP-dependent RNA helicase DDX4; Vasa homolog; VASA; VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4