Human VAP-1/AOC3 Antibody

Catalog #: AF3957 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3957
AF3957-SP

Detection of Human VAP‑1/AOC3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VAP-1/AOC3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VAP‑1/AOC3 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse VAP-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VAP‑1/AOC3
Gly27-Asn763
Accession # Q16853

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human VAP‑1/AOC3 by Western Blot. Western blot shows lysates of human lung tissue, human kidney tissue, human liver tissue, and human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VAP-1/AOC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3957) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAP-1/AOC3 at approximately 95-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human VAP‑1/AOC3 by Simple Western^TM. Simple Western lane view shows lysates of human lung tissue and heart tissue, loaded at 0.2 mg/mL. A specific band was detected for VAP-1/AOC3 at approximately 123 kDa (as indicated) using 10 µg/mL of Goat Anti-Human VAP-1/AOC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3957) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VAP-1/AOC3

Vascular Adhesion Protein-1 (VAP-1) is a copper amine oxidase with a topaquinone cofactor. VAP-1 is a Type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases (1, 2). VAP-1 catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H₂O₂ (3). The enzyme is sensitive to inhibition by semicarbazide. VAP-1 expression is highest in the endothelium of lung, heart, and intestine, but low in tissues such as brain, spleen, kidney, and liver (4). VAP-1 vascular expression is regulated at sites of inflammation through its release from intracellular granules in which the protein is stored (5). The adhesive function of VAP-1 has been demonstrated in studies showing that the protein is important for the adherence of certain lymphocyte subtypes to inflamed endothelial tissues (6). VAP-1 mediated adhesion is involved in the process of leukocyte extravasation, an important feature of inflammatory responses. The role of VAP-1 amine oxidase activity in this process is not fully defined, but it appears to be carbohydrate-dependent (7). VAP-1 is considered to be a therapeutic target for diabetes, oxidative stress, and inflammatory diseases (8).

References

Kurkijärvi, R. et al. (1998) J. Immunol. 161:1549.
Gearing, A.J.H. and W. Newman (1993) Immunol. Today 14:506.
Lizcano, J.M. et al. (1998) Biochem. J. 331:69.
Smith, D.J. et al. (1998) J. Exp. Med. 188:17.
Jaakkala K. et al. (2000) Am. J. Pathol. 157:463.
Salmi, M. and J. Jalkanen (2001) Trends Immunol. 22:211.
Salmi, M. and J. Jalkanen (1996) J. Exp. Med. 183:569.
Dunkel, P. et al. (2008) Curr. Med. Chem. 15:1827.

Long Name

Vascular Adhesion Protein-1

Entrez Gene IDs

8639 (Human); 11754 (Mouse); 29473 (Rat)

Alternate Names

amine oxidase, copper containing 3 (vascular adhesion protein 1); AOC3; Copper amine oxidase; EC 1.4.3; HPAO; HPAOSSAO; Semicarbazide-sensitive amine oxidase; SSAO; VAP1; VAP-1; VAP1EC 1.4.3.21; VAP-1membrane primary amine oxidase; Vascular adhesion protein 1