Human ULBP-4/RAET1E Antibody Summary
Gly30-Asp225
Accession # Q8TD07
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human ULBP‑4/RAET1E by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ULBP-4/ RAET1E Monoclonal Antibody (Catalog # MAB6285) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for ULBP-4/RAET1E at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of ULBP‑4/RAET1E in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human ULBP-4/RAET1E Monoclonal Antibody (Catalog # MAB6285, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).
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ULBP‑4/RAET1E in Human Ovarian Cancer Tissue. ULBP-4/RAET1E was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human ULBP-4/ RAET1E Monoclonal Antibody (Catalog # MAB6285) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of ULBP‑4/RAET1E in U87-MG cells by Flow Cytometry. U87-MG cells were stained with Mouse Anti-Human ULBP‑4/RAET1E Monoclonal Antibody (Catalog # MAB6285, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
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Detection of ULBP‑4/RAET1E in HEK293 cells transfected with Human ULBP-4 (A) vs irrelevant (B) by Flow Cytometry HEK293 cells transfected with Human ULBP-4 (A) vs irrelevant (B) were stained with Mouse Anti-Human ULBP‑4/RAET1E Monoclonal Antibody (Catalog # MAB6285) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ULBP-4/RAET1E
ULBP-4 (cytomegalovirus glycoprotein UL16 binding protein 4), also called RAET1E (retinoic acid early transcript 1E), Letal (lymphocyte effector cell toxicity activating ligand) and NKG2DL4 (NKG2D ligand 4), is a 40-50 kDa member of the ULBP/RAET1 family of cell surface proteins that function as ligands for NKG2D (1‑6). While most family members are GPI-anchored, only ULBP-4/RAET1E and ULBP-5/RAET1G express a transmembrane form (1, 4, 6, 7). Human ULBP-4 mRNA encodes 263 amino acids (aa) including a 30 aa signal sequence, a 195 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 15 aa cytoplasmic sequence. A soluble 35 kDa form diverges at aa 208 and is thought to antagonize the transmembrane form (5). Other potential splice variants of 220, 227 and 280 aa are transmembrane proteins (8). Within the ECD, ULBP-4 shares 34-41% aa sequence identity with family members (1, 7). Rodent NKG2D ligands Rae-1 alpha -epsilon are, like human ULBP and MIB proteins, distantly related to MHC class I proteins, but none of the families share significant sequence identity (2, 4). Low expression of ULBP‑4 mRNA is found in normal tissues, with high expression variably reported in the small intestine (3) and skin (4). Expression is stimulated by TNF-alpha and down‑regulated by retinoic acid (3). ULBP-4 is abnormally expressed on most colon cancer and some other tumor cell lines and virus-infected peripheral blood cells (3, 6). ULBP-4 binds and costimulates NKG2D-expressing effector cells including NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells, activating cytolytic activity and/or cytokine production (3, 4, 7). In some gamma δ T cells, direct ULBP-4 binding to both TCR gamma δ and NKG2D has been demonstrated (6). ULBP-4 is also thought to function as a minor histocompatibility antigen in humans (1).
- Radosavljevic, M. et al. (2002) Genomics 79:114.
- Kondo, M. et al. (2010) Immunogenetics 62:441.
- Conejo-Garcia, J.R. et al. (2003) Cancer Biol. Ther. 2:446.
- Chalupny, N.J. et al. (2003) Biochem. Biophys. Res. Commun. 305:129.
- Cao, W. et al. (2007) J. Biol. Chem. 282:18922.
- Kong, Y. et al. (2009) Blood 114:310.
- Bacon, L. et al. (2004) J. Immunol. 173:1078.
- Cao, W. et al. (2008) Int. Immunol. 20:981.
Product Datasheets
Citations for Human ULBP-4/RAET1E Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The NKG2D ligand ULBP4 is not expressed by human monocytes
Authors: M Lazarova, Y Kim, A Steinle
PLoS ONE, 2021-02-08;16(2):e0246726.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Clinicopathological relevance of tumor expression of NK group 2 member D ligands in resected non-small cell lung cancer
Authors: R Okita, A Maeda, K Shimizu, Y Nojima, S Saisho, M Nakata
Oncotarget, 2019-11-26;10(63):6805-6815.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Targeting chemotherapy-resistant leukemia by combining DNT cellular therapy with conventional chemotherapy
Authors: B Chen, JB Lee, H Kang, MD Minden, L Zhang
J. Exp. Clin. Cancer Res., 2018-04-24;37(1):88.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells
Authors: C Breunig, J Pahl, M Küblbeck, M Miller, D Antonelli, N Erdem, C Wirth, R Will, A Bott, A Cerwenka, S Wiemann
Cell Death Dis, 2017-08-03;8(8):e2973.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Adoptively transferred V?9V?2 T cells show potent antitumor effects in a preclinical B cell lymphomagenesis model
Authors: NA Zumwalde, A Sharma, X Xu, S Ma, CL Schneider, JC Romero-Mas, AW Hudson, A Gendron-Fi, SC Kenney, JE Gumperz
JCI Insight, 2017-07-06;2(13):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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