Human Ubiquitin+1 Antibody

Catalog #: MAB7031 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7031
MAB7031-SP

All Ubiquitin+1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ubiquitin+1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ubiquitin+1 in ELISAs and Western blots. In in ELISAs and Western blots, no cross-reactivity with Ubiquitin is observed.

Source

Monoclonal Mouse IgG_2B Clone # 83456

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Ubiquitin+1
Met1-Gly75, YADLREDPDRQDHHPGSGAQ (C-terminal sequence generated by a frameshift)
Accession # CAA44911

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Ubiquitin+1 (Catalog # 703-UB)

Human Ubiquitin+1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human Ubiquitin+1 Biotinylated Antibody (Catalog # BAM7032)

Standard: Recombinant Human Ubiquitin+1 Protein, CF (Catalog # 703-UB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ubiquitin+1

Ubiquitin+1 has a carboxyl terminal amino acid sequence that differs from normal Ubiquitin. The different carboxyl terminal sequence appears to result from a frameshift in the Ubiquitin mRNA. The underlying mechanisms creating the mRNA frameshift are not clearly understood. The occurrence of the frameshift that generates Ubiquitin+1 is much more prevalent in patients with Alzheimers Disease or with Down Syndrome than in control individuals who are not afflicted with the disorders. The monoclonal anti-Ubiquitin+1 (Catalog # MAB703) and rabbit polyclonal anti-Ubiquitin+1 (Catalog # AF703) antibodies were raised against the Ubiquitin+1 carboxyl terminal sequence that differs from normal Ubiquitin and are therefore non-reactive with Ubiquitin. Monoclonal anti-Ubiquitin (Catalog # MAB701) detects both Ubiquitin and Ubiquitin+1 indicating that the epitope recognized by this antibody is contained in the portion of the proteins that are identical (1, 2).

References

van Leeuwen, F.W. et al. (1998) Science 279:242.
van Leeuwen, F.W. et al. (1998) Trends Neurosci. 21:331.

Long Name

frame shift mutant

Entrez Gene IDs

7314 (Human); 298693 (Rat)

Alternate Names

FLJ25987; MGC8385; polyubiquitin B; polyubiquitin-B; RPS27A; UBA52; UBC; ubiquitin B; Ubiquitin+1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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