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Human TWEAK R/TNFRSF12 Antibody

Catalog #: MAB1199
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1199
MAB1199-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TWEAK R/TNFRSF12 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TWEAK R/TNFRSF12 in direct ELISAs and Western blots. Shows approximately 40% cross-reactivity with recombinant mouse TWEAK R and no cross-reactivity with recombinant human (rh) OPG or rhTNF RI.
Source
Monoclonal Rat IgG2A Clone # 314502
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TWEAK R/TNFRSF12
Glu28-Trp79
Accession # Q9NP84.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1199-TW)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TWEAK R/TNFRSF12

The gene for TNF-related weak inducer of apoptosis receptor (TWEAK R) was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Human TWEAK R cDNA encodes a 129 amino acid (aa) residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1-3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain conatins one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death, cell proliferation, and angiogenesis (2-6). In new born mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

References
  1. Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
  2. Feng, S. et al. (2000) Am J. Pathol. 156:1253.
  3. Wiley, S. et al. (2001) Immunity 15:837.
  4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
  5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
  6. Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.
Long Name
TNF-like and Weak Inducer of Apoptosis Receptor
Entrez Gene IDs
51330 (Human); 27279 (Mouse)
Alternate Names
CD266 antigen; CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Fn14; FN14tumor necrosis factor receptor superfamily member 12A; TNFRSF12; TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; TWEAK R; TWEAKR; tweak-receptor; type I transmembrane protein Fn14

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Citations for Human TWEAK R/TNFRSF12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Transcriptomic analysis reveals cell apoptotic signature modified by heparanase in melanoma cells
    Authors: T Song, D Spillmann
    J. Cell. Mol. Med., 2019-05-02;0(0):.
  2. A Homogeneous HTRF Assay for the Identification of Inhibitors of the TWEAK-Fn14 Protein Interaction
    Authors: Tiziana Benicchi, Sara Iozzi, Andreas Svahn, Hanna Axelsson, Elisa Mori, Simonetta Bernocco et al.
    J Biomol Screen

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