Human Tryptase gamma-1/TPSG1 Antibody

Catalog #: MAB1667 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1667
MAB1667-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tryptase gamma-1/TPSG1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tryptase gamma ‑1/TPSG1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) MCPT-1, recombinant human (rh) Tryptase alpha, rmTryptase beta -1, rhTryptase beta -2, or rmTryptase-5 is observed.

Source

Monoclonal Rat IgG_2B Clone # 279005

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tryptase gamma ‑1/TPSG1
Arg20-Arg281
Accession # Q9NRR2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Tryptase gamma ‑1/TPSG1 (Catalog # 1667-SE)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Tryptase gamma ‑1/TPSG1 (Catalog # 1667-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase gamma-1/TPSG1

As mediators of inflammatory and allergic response, mast cells are found throughout the body concentrated near blood vessels in connective tissue and the mucous membranes of the respiratory and gastrointestinal tract (3). Upon activation, human mast cells release granules that are enriched with neutral serine proteases including tryptases, chymase and cathepsin G (4). Tryptase gamma -1, also called transmembrane tryptase, is encoded by TPSG1, one of many serine protease genes clustered in human chromosome 16p13.3 (5). Human Tryptase gamma ‑1 is synthesized as a 321 amino acid preproenzyme with a C-terminal transmembrane anchor (1, 2).

References

Wong, G.W. et al. (1999) J. Biol. Chem. 274:30784.
Caughey, G.H. et al. (2000) J. Immunol. 164:6566.
Harris, J.L. et al. (2001) J. Biol. Chem. 276:34941.
Miller, H.R.P. and A.D. Pemberton (2002) Immunology 105:375.
Wong, G.W. et al. (2002) J. Biol. Chem. 277:41906.

Entrez Gene IDs

25823 (Human)

Alternate Names

EC 3.4.21; EC 3.4.21.-; gamma I; gamma II; lung tryptase; mast cell protease II; mast cell tryptase; PRSS31skin tryptase; Serine protease 31; TMTpituitary tryptase; TPSG1; Transmembrane tryptase; trpA; tryptase gamma 1; tryptase gamma I; tryptase gamma II; tryptase gamma; Tryptase gamma1; Tryptase gamma-1