Home / Trypsin 1/PRSS1 / Human Trypsin 1/PRSS1 Antibody

Human Trypsin 1/PRSS1 Antibody

Catalog #: AF3848
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3848
AF3848-SP
Trypsin 1/PRSS1 in Human Pancreas.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Trypsin 1/PRSS1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Trypsin 1/PRSS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Trypsin 2 and rhTrypsin 3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Trypsin 1/PRSS1
Ala16-Ser247
Accession # P07477
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Trypsin 1/PRSS1 (Catalog # 3848-SE)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Trypsin 1/PRSS1 (Catalog # 3848-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Trypsin 1/PRSS1 antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

Trypsin 1/PRSS1 in Human Pancreas. Trypsin 1/PRSS1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Trypsin 1/PRSS1 Monoclonal Antibody (Catalog # MAB3848) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trypsin 1/PRSS1

Human Trypsin 1, encoded by the PRSS1 gene, is also known as Cationic Trypsinogen (1). Constituting approximately two-thirds of the total trypsin content in normal pancreatic juice, it is the most abundant trypsin isoform produced by the pancreas. It contains a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 1 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro-enzymes (2). Mutations in the PRSS1 gene can cause hereditary pancreatitis (3).

References
  1. Emi, M. et al. (1986) Gene 41:305.
  2. Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1483, Academic Press, San Diego.
  3. Teich, N. et al. (2006) Hum. Mutat. 27:721.
Entrez Gene IDs
5644 (Human); 114228 (Mouse); 24691 (Rat)
Alternate Names
Beta-trypsin; Cationic trypsinogen; digestive zymogen; DKFZp779A0837; EC 3.4.21; EC 3.4.21.4; FLJ57296; MGC120175; MGC149362; nonfunctional trypsin 1; protease, serine, 1 (trypsin 1); PRSS1; PTRYI; Serine protease 1; TRP1; TRY1beta-trypsin; TRY4; Trygn16; TRYP1; Trypsin 1; Trypsin I; trypsin-1; Trypsinogen 1; trypsinogen A

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Citations for Human Trypsin 1/PRSS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Robust autoactivation, chymotrypsin C independence and diminished secretion define a subset of hereditary pancreatitis-associated cationic trypsinogen mutants
    Authors: Andrea Geisz, Péter Hegyi, Miklós Sahin-Tóth
    FEBS Journal
  2. SARS-CoV-2 infection induces beta cell transdifferentiation
    Authors: Tang X, Uhl S, Zhang T et al.
    Cell Metabolism
  3. Intragenic duplication: a novel mutational mechanism in hereditary pancreatitis
    Authors: Maiken T. Joergensen, Andrea Geisz, Klaus Brusgaard, Ove B. Schaffalitzky Schaffalitzky de Muckadell, Péter Hegyi, Anne-Marie Gerdes et al.
    Pancreas
  4. Expression of human cationic trypsinogen (PRSS1) in murine acinar cells promotes pancreatitis and apoptotic cell death
    Authors: T Athwal, W Huang, R Mukherjee, D Latawiec, M Chvanov, R Clarke et al.
    Cell Death & Disease
  5. Pathogenic cellular role of the p.L104P human cationic trypsinogen variant in chronic pancreatitis
    Authors: Anita Balázs, Péter Hegyi, Miklós Sahin-Tóth
    American Journal of Physiology-Gastrointestinal and Liver Physiology
  6. Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity
    Authors: PR Tata, RD Chow, SV Saladi, A Tata, A Konkimalla, A Bara, D Montoro, LP Hariri, AR Shih, M Mino-Kenud, H Mou, S Kimura, LW Ellisen, J Rajagopal
    Dev. Cell, 2018-03-26;44(6):679-693.e5.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Functional effects of 13 rare PRSS1 variants presumed to cause chronic pancreatitis
    Authors: Andrea Schnúr, Sebastian Beer, Heiko Witt, Péter Hegyi, Miklós Sahin-Tóth
    Gut

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