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Human TrkC Antibody

Catalog #: AF373
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF373
AF373-SP
Detection of HAF017 TrkC by Western Blot.
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Citations (7)
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Human TrkC Antibody Summary

Species Reactivity
Human
Specificity
Detects human TrkC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human TrkB is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TrkC
Cys32-Asp428
Accession # Q96CY4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
Human TrkC transfected Baf/3 cells; A549 human lung carinoma cell line
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human brain (hippocampus) and perfusion fixed frozen sections of mouse brain (cortex)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human NT-3 (Catalog # 267-N3) to immobilized Recombinant Human TrkC Fc Chimera (Catalog # 373-TC) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of HAF017 TrkC antibody by Western Blot. View Larger

Detection of HAF017 TrkC by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human brain (hippocampus) tissue, human brain (hypothalamus) tissue, and human brain stem tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF373) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TrkC at approximately 140, 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human TrkC antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human TrkC by Simple WesternTM. Simple Western lane view shows lysates of human brain (hypothalamus) tissue, loaded at 0.2 mg/mL. A specific band was detected for TrkC at approximately 164 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF373) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Flow Cytometry View Larger

Detection of TrkC in A549 cells by Flow Cytometry A549 cells were stained with Goat Anti-Human TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF373, filled histogram) or isotype control antibody (Catalog # 4-001-A, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkC

The neurotrophins, including NGF, BDNF, NT-3 and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). P75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low-affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT‑3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1, 2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, Trks are highly conserved between species with the extracellular domains of human and mouse TrkC's showing 94% amino acid sequence identity (5). The proteins also exhibit cross-species activity. The primary location of TrkC expression is in the nervous system and, specifically, in regions of the CNS. Low level TrkC expression has also been observed in a wide variety of tissues outside the nervous system (6).

References
  1. Huang, E.J. and L.F. Reichardt. (2003) Annu. Rev. Biochem. 72:(epub ahead of print).
  2. Dechant, G. (2001) Cell Tissue Res. 305:229.
  3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
  4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  5. Menn, B. et al. (1998) J. Comp. Neurol. 401:47.
  6. Shelton, D. et al. (1995) J. Neurosci. 15:477.
Long Name
Neurotrophic Tyrosine Kinase Receptor C
Entrez Gene IDs
4916 (Human); 18213 (Mouse); 29613 (Rat)
Alternate Names
EC 2.7.10; EC 2.7.10.1; ETS related protein-neurotrophic receptor tyrosine kinase fusion protein; ETV6-NTRK3 fusion; gp145(trkC); GP145-TrkC; Neurotrophic tyrosine kinase receptor type 3; neurotrophic tyrosine kinase, receptor, type 3; NT 3 receptor; NT-3 growth factor receptor; NTRK3; TrkC tyrosine kinase; TrkC; trk-C; TRKCneurotrophin 3 receptor; tyrosine kinase receptor C

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Citations for Human TrkC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Isolation of human pluripotent stem cell-derived sensory neuron subtypes by immunopanning
    Authors: Kenyi Saito-Diaz, Christina James, Archie Jayesh Patel, Nadja Zeltner
    Frontiers in Cell and Developmental Biology
  2. In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system
    Authors: M Badiola-Ma, T Osaki, RD Kamm, J Samitier
    Scientific Reports, 2022-12-09;12(1):21318.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Selective activation and down‐regulation of Trk receptors by neurotrophins in human neurons co‐expressing TrkB and TrkC
    Authors: Sarah Ateaque, Spyros Merkouris, Sean Wyatt, Nicholas D. Allen, Jia Xie, Peter S. DiStefano et al.
    Journal of Neurochemistry
  4. Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia
    Authors: Ugomma C. Eze, Aparna Bhaduri, Maximilian Haeussler, Tomasz J. Nowakowski, Arnold R. Kriegstein
    Nature Neuroscience
  5. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
    Authors: Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S
    Nat Biotechnol, 2014-11-10;33(1):89-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  6. TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
    Authors: Huang YT, Lai PC, Wu CC
    Int. J. Oncol., 2010-10-01;37(4):943-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  7. BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
    Authors: Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
    Int. J. Oncol., 2010-06-01;36(6):1469-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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