Human Transglutaminase 4/TGM4 Biotinylated Antibody

Catalog #: BAF5760 Datasheet / COA / SDS

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BAF5760

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Human Transglutaminase 4/TGM4 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Transglutaminase 4 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Transglutaminase 2, rhTransglutaminase 3, rhTransglutaminase 7, and recombinant mouse Transglutaminase 2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 4/TGM4
Met2-Lys684
Accession # AAC50516

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 4/TGM4

TGM4 (Transglutaminase 4; also TGP and Prostate-specific transglutaminase) is a 77 kDa member of the papain-like family of transglutaminases. It is expressed by various tumor cell lines plus prostatic epithelium, and may participate in semen protein modification. TGM4, like other TGMs, can apparently mediate transamidation, plus esterification reactions. Human TGM4 is 684 amino acids (aa) in length. Although it does not contain a signal sequence, based on rodent, it is likely secreted as a homodimer. TGM4 contains an active enzyme site between aa 263‑353, and shows a C-terminal Ig-like domain (aa 587‑684). The molecule has a requirement for divalent calcium. There are two potential splice variants. One shows a 45 aa insertion after Leu8, while another shows a 12 aa substitution for aa 1‑80. Human full‑length TGM4 shares only 54% aa identity with mouse TGM4.

Entrez Gene IDs

7047 (Human); 331046 (Mouse); 64679 (Rat)

Alternate Names

EC 2.3.2.13; Fibrinoligase; hTGP; Prostate transglutaminase; Prostate-specific transglutaminase; protein-glutamine gamma-glutamyltransferase 4; TG(P); TGase P; TGase-4; TGM4; TGP; TGPFLJ26776; transglutaminase 4 (prostate); Transglutaminase 4; Transglutaminase P; transglutaminase-4