Human Transglutaminase 4/TGM4 Antibody

Catalog #: AF5760 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5760
AF5760-SP

Transglutaminase 4/TGM4 in Human Prostate.

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Human Transglutaminase 4/TGM4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Transglutaminase 4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Transglutaminase 2, rhTransglutaminase 3, rhTransglutaminase 7, and recombinant mouse Transglutaminase 2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 4
Met2-Lys684
Accession # AAC50516

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Transglutaminase 4/TGM4 in Human Prostate. Transglutaminase 4/TGM4 was detected in immersion fixed paraffin-embedded sections of human prostate using Sheep Anti-Human Transglutaminase 4/TGM4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5760) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot

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Detection of Human Transglutaminase 4/TGM4 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Transglutaminase 4/TGM4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5760) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Transglutaminase 4/TGM4 at approximately 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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Transglutaminase 4/TGM4 in Human Prostate. Transglutaminase 4/TGM4 was detected in paraffin-embedded sections of human prostate using Sheep Anti-Human Transglutaminase 4/TGM4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5760) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 4/TGM4

TGM4 (Transglutaminase 4; also TGP and Prostate-specific transglutaminase) is a 77 kDa member of the papain-like family of transglutaminases. It is expressed by various tumor cell lines plus prostatic epithelium, and may participate in semen protein modification. TGM4, like other TGMs, can apparently mediate transamidation, plus esterification reactions. Human TGM4 is 684 amino acids (aa) in length. Although it does not contain a signal sequence, based on rodent, it is likely secreted as a homodimer. TGM4 contains an active enzyme site between aa 263‑353, and shows a C-terminal Ig-like domain (aa 587‑684). The molecule has a requirement for divalent calcium. There are two potential splice variants. One shows a 45 aa insertion after Leu8, while another shows a 12 aa substitution for aa 1‑80. Human full‑length TGM4 shares only 54% aa identity with mouse TGM4.

Entrez Gene IDs

7047 (Human); 331046 (Mouse); 64679 (Rat)

Alternate Names

EC 2.3.2.13; Fibrinoligase; hTGP; Prostate transglutaminase; Prostate-specific transglutaminase; protein-glutamine gamma-glutamyltransferase 4; TG(P); TGase P; TGase-4; TGM4; TGP; TGPFLJ26776; transglutaminase 4 (prostate); Transglutaminase 4; Transglutaminase P; transglutaminase-4