Human Transglutaminase 2/TGM2 Antibody

Catalog #: MAB4376 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4376
MAB4376-SP

Detection of Human Transglutaminase 2/TGM2 by Western Blot.

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Human Transglutaminase 2/TGM2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Transglutaminase 2/TGM2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Transglutaminase 3, 4, 7, or recombinant mouse Transglutaminase 2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 716620

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 2/TGM2
Ala2-Ala687 (Asn533Thr, Leu655Val)
Accession # P21980

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Transglutaminase 2/TGM2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Transglutaminase 2/TGM2 Monoclonal Antibody (Catalog # MAB4376) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Transglutaminase 2/TGM2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 2/TGM2

Transglutaminase 2 (TG2), encoded by the TGM2 gene, is also known as tissue transglutaminase (tTG), transgluytaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1‑3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6‑7).

References

Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
Chen, J.S.K. and K. Mehta (1999) Internat. J. Biochem. Cell Biol. 31:817.
Griffin, M. et al. (2002) Biochem. J. 368:377.
Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.

Entrez Gene IDs

7052 (Human); 21817 (Mouse); 56083 (Rat)

Alternate Names

C polypeptide; EC 2.3.2.13; G-ALPHA-h; protein-glutamine gamma-glutamyltransferase 2; protein-glutamine-gamma-glutamyltransferase; TG(C); TG2; TGase C; TGase H; TGase-2; TGase-H; TGC; TGCGNAH; TGM2; Tissue transglutaminase; transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase); Transglutaminase 2; Transglutaminase C; Transglutaminase H; transglutaminase-2; tTG