Human TRA-1-85/CD147 PE-conjugated Antibody

Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA-1-85/CD147 PE-conjugated Monoclonal Antibody (Catalog # FAB3195P) or (B) Mouse IgG₁Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Detection of Mouse TRA-1-85/CD147 by Flow Cytometry Flow cytometry analysis of total cell fractions of dissociated cells from PDX models.A) Basal-like xenograft cells. B) Luminal-like xenograft cells. A and B displays pseudo-color dot plots (left panels) and histograms (right panels). Freshly harvested xenografts were minced and the whole cell suspensions were washed and stained with monoclonal antibody towards EpCAM, TRA-1-85 (filled blue in histograms), H2-kd (red line in lower histogram) and Hoecst-33342 (intensity measure for DNA content of cells, grey contours in both histograms. Left peak indicate mouse cells, right peak indicate human cells). The population positive for both EpCAM and TRA-1-85, i.e the human tumor cells, are indicated with a circle in the dot plots. C) Flow cytometry analysis of double stained samples (marker of interest and EpCAM/Tra-1-85) of the Luminal-like PDX model. Flow cytometry histograms show the distribution of the markers indicated in the figure. Filled blue histogram represents EpCAM positive tumor cell population, and the EpCAM negative population (mouse stroma cells) is indicated by the red line. Grey contour represent unstained control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25419568), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse TRA-1-85/CD147 by Flow Cytometry Flow cytometry analysis of total cell fractions of dissociated cells from PDX models.A) Basal-like xenograft cells. B) Luminal-like xenograft cells. A and B displays pseudo-color dot plots (left panels) and histograms (right panels). Freshly harvested xenografts were minced and the whole cell suspensions were washed and stained with monoclonal antibody towards EpCAM, TRA-1-85 (filled blue in histograms), H2-kd (red line in lower histogram) and Hoecst-33342 (intensity measure for DNA content of cells, grey contours in both histograms. Left peak indicate mouse cells, right peak indicate human cells). The population positive for both EpCAM and TRA-1-85, i.e the human tumor cells, are indicated with a circle in the dot plots. C) Flow cytometry analysis of double stained samples (marker of interest and EpCAM/Tra-1-85) of the Luminal-like PDX model. Flow cytometry histograms show the distribution of the markers indicated in the figure. Filled blue histogram represents EpCAM positive tumor cell population, and the EpCAM negative population (mouse stroma cells) is indicated by the red line. Grey contour represent unstained control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25419568), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse TRA-1-85/CD147 by Flow Cytometry Flow cytometry analysis of total cell fractions of dissociated cells from PDX models.A) Basal-like xenograft cells. B) Luminal-like xenograft cells. A and B displays pseudo-color dot plots (left panels) and histograms (right panels). Freshly harvested xenografts were minced and the whole cell suspensions were washed and stained with monoclonal antibody towards EpCAM, TRA-1-85 (filled blue in histograms), H2-kd (red line in lower histogram) and Hoecst-33342 (intensity measure for DNA content of cells, grey contours in both histograms. Left peak indicate mouse cells, right peak indicate human cells). The population positive for both EpCAM and TRA-1-85, i.e the human tumor cells, are indicated with a circle in the dot plots. C) Flow cytometry analysis of double stained samples (marker of interest and EpCAM/Tra-1-85) of the Luminal-like PDX model. Flow cytometry histograms show the distribution of the markers indicated in the figure. Filled blue histogram represents EpCAM positive tumor cell population, and the EpCAM negative population (mouse stroma cells) is indicated by the red line. Grey contour represent unstained control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25419568), licensed under a CC-BY license. Not internally tested by R&D Systems.