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Human TNF RI/TNFRSF1A Antibody

Catalog #: MAB625
27 Citations 2 Reviews Datasheet / COA / SDS
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MAB625-SP
MAB625-500
MAB625-100
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TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody.
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Citations (27)
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Reviews (2)
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Human TNF RI/TNFRSF1A Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF RI/TNFRSF1A in ELISAs and Western blots. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) TGF-alpha, hTGF-beta 1, porcine (p) TGF-beta 1, rhTGF-beta 1, pTGF-beta 1.2, pTGF-beta 2, recombinant chicken TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-beta, or rhTNF RII is observed.
Source
Monoclonal Mouse IgG1 Clone # 16805
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human TNF RI/TNFRSF1A
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)
under non-reducing conditions only

Human TNF RI/TNFRSF1A Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human TNF RI/TNFRSF1A Biotinylated Antibody (Catalog # BAF225)

Standard: Recombinant Human sTNF RI/TNFRSF1A Protein (Catalog # 636-R1)

Neutralization
Measured by its ability to neutralize TNF RI/TNFRSF1A-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-6 µg/mL in the presence of 0.3 μg/mL Recombinant Human TNF RI/TNFRSF1A, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody. View Larger

TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody. Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1) inhibits Recombinant Human TNF-a (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB625). The ND50 is typically 1-6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

 

Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

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Citations for Human TNF RI/TNFRSF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. TNF? counteracts interleukin-10 anti-inflammatory pathway through the NOX2-Lyn-SHP-1 axis in human monocytes
    Authors: Ben-Khemis, M;Liu, D;Pintard, C;Song, Z;Hurtado-Nedelec, M;Marie, JC;El-Benna, J;Dang, PM;
    Redox biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. TSPO acts as an immune resistance gene involved in the T cell mediated immune control of glioblastoma
    Authors: Menevse, AN;Ammer, LM;Vollmann-Zwerenz, A;Kupczyk, M;Lorenz, J;Weidner, L;Hussein, A;Sax, J;M�hlbauer, J;Heuschneider, N;Rohrmus, C;Mai, LS;Jachnik, B;Stamova, S;Volpin, V;Durst, FC;Sorrentino, A;Xydia, M;Milenkovic, VM;Bader, S;Braun, FK;Wetzel, C;Albert, NL;Tonn, JC;Bartenstein, P;Proescholdt, M;Schmidt, NO;Linker, RA;Riemenschneider, MJ;Beckhove, P;Hau, P;
    Acta neuropathologica communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Inflammation-Driven Regulation of PD-L1 and PD-L2, and Their Cross-Interactions with Protective Soluble TNFalpha Receptors in Human Triple-Negative Breast Cancer
    Authors: T Baram, N Oren, N Erlichman, T Meshel, A Ben-Baruch
    Cancers, 2022-07-19;14(14):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  4. ST6Gal-I-mediated sialylation of the epidermal growth factor receptor modulates cell mechanics and enhances invasion
    Authors: TC Rao, RR Beggs, KE Ankenbauer, J Hwang, VP Ma, K Salaita, SL Bellis, AL Mattheyses
    The Journal of Biological Chemistry, 2022-02-12;0(0):101726.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. High Glucose Exacerbates TNF-&alpha-Induced Proliferative Inhibition in Human Periodontal Ligament Stem Cells through Upregulation and Activation of TNF Receptor 1
    Authors: W Zhu, Q Qiu, H Luo, F Zhang, J Wu, X Zhu, M Liang
    Stem Cells Int, 2020-02-05;2020(0):4910767.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. TNF-alpha Drives the CCL4 Expression in Human Monocytic Cells: Involvement of the SAPK/JNK and NF-kappa B Signaling Pathways
    Authors: Rasheed Ahmad, Shihab Kochumon, Betty Chandy, Steve Shenouda, Merin Koshy, Amal Hasan et al.
    Cellular Physiology and Biochemistry
  7. ANKRD1 regulates innate immune responses against herpes simplex virus 1: A potential role in eczema herpeticum
    Authors: L Bin, X Li, B Richers, JE Streib, JW Hu, P Taylor, DYM Leung
    J. Allergy Clin. Immunol., 2018-01-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralizing
  8. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes
    Sci Rep, 2016-09-27;6(0):34310.
    Species: Human
    Sample Types:
  9. Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1.
    Authors: Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C
    J Biol Chem, 2014-12-23;290(7):4022-37.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: ELISA Development (Capture)
  10. Inhibiting TNF-α signaling does not attenuate induction of endotoxin tolerance
    Authors: Christopher Loosbroock, Christopher Loosbroock
    Journal of Inflammation Research
  11. A short-term treatment with tumor necrosis factor-alpha enhances stem cell phenotype of human dental pulp cells
    Authors: Mayu Ueda, Takuo Fujisawa, Mitsuaki Ono, Emilio Satoshi Hara, Hai Thanh Pham, Ryu Nakajima et al.
    Stem Cell Research & Therapy
  12. Interferon-gamma and tumor necrosis factor-alpha act synergistically to up-regulate tissue factor in alveolar epithelial cells
    Authors: Julie A. Bastarache, Sara C. Sebag, Brandon S. Grove, Lorraine B. Ware
    Experimental Lung Research
  13. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
    Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C
    Mucosal Immunol, 2012-06-13;5(0):702.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012-02-17;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  15. Inflammatory Cytokines IL-32 and IL-17 Have Common Signaling Intermediates despite Differential Dependence on TNF-Receptor 1
    Authors: Emily Turner-Brannen, Ka-Yee Grace Choi, Ryan Arsenault, Hani El-Gabalawy, Scott Napper, Neeloffer Mookherjee
    The Journal of Immunology
  16. IRF5 is required for late-phase TNF secretion by human dendritic cells.
    Authors: Krausgruber T, Saliba D, Ryzhakov G
    Blood, 2010-03-17;115(22):4421-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  18. Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
    Authors: Emsley HC, Smith CJ, Gavin CM
    BMC Neurol, 2007-02-28;7(0):5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  19. Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1.
    Authors: Lee SY, Cherla RP, Tesh VL
    Infect. Immun., 2006-12-28;75(3):1291-302.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  20. LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis.
    Authors: Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A
    Arthritis Rheum., 2006-05-01;54(5):1451-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  22. Absence of caspase 8 and high expression of PED protect primitive neural cells from cell death.
    Authors: Ricci-Vitiani L, Pedini F, Mollinari C, Condorelli G, Bonci D, Bez A, Colombo A, Parati E, Peschle C, De Maria R
    J. Exp. Med., 2004-11-15;200(10):1257-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1).
    Authors: Chopin V, Slomianny C, Hondermarck H, Le Bourhis X
    Exp. Cell Res., 2004-08-15;298(2):560-73.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004-01-01;284(1):99-106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Array Development
  25. Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
    Authors: Gee K, Lim W, Ma W, Nandan D, Diaz-Mitoma F, Kozlowski M, Kumar A
    J. Immunol., 2002-11-15;169(10):5660-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Innate immunity associated with inflammatory responses and cytokine production against common dietary proteins in patients with autism spectrum disorder.
    Authors: Jyonouchi H, Sun S, Itokazu N
    Neuropsychobiology, 2002-01-01;46(2):76-84.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  27. A pilot study of pentoxifylline for the treatment of primary sclerosing cholangitis.
    Authors: Bharucha AE, 2019, Jorgensen R, Lichtman SN, LaRusso NF, Lindor KD
    2000-09-01;95(9):2338-42.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

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Reviews for Human TNF RI/TNFRSF1A Antibody

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Human TNF RI/TNFRSF1A Antibody
By Anonymous on 12/15/2017
Application: B/N Sample Tested: Cell culture supernatant Species: Human
Human TNF RI/TNFRSF1A Antibody
By Anonymous on 11/22/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used MAB625 as the solid phase antibody, 636-R1 as the immunoassay standard and BAF225 as the detection antibody in a sandwich ELISA. Everything worked very well.

ELISA Human TNF RI/TNFRSF1A Antibody MAB625