Human TMPRSS2 Antibody

Catalog #: MAB107231 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB107231-100
MAB107231-SP

Detection of TMPRSS2 in HEK293 Human Cell Line Transfected with Human TMPRSS2 and eGFP by Flow Cytometry.

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All TMPRSS2 Antibodies

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Human TMPRSS2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TMPRSS2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1038105

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human TMPRSS2 synthetic peptide
Accession # O15393

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human TMPRSS2 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TMPRSS2 in HEK293 Human Cell Line Transfected with Human TMPRSS2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human TMPRSS2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human TMPRSS2 Monoclonal Antibody (Catalog # MAB107231) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMPRSS2

TMPRSS2 belongs to the serine protease family. It contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. TMPRSS2 facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms: proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein, which activates the glycoprotein for cathepsin Lindependent host cell entry. Alternatively spliced transcripts encoding different proteins have been described.

Long Name

Transmembrane protease, serine 2

Entrez Gene IDs

7113 (Human); 50528 (Mouse); 102137447 (Cynomolgus Monkey)

Alternate Names

EC 3.4.21; Epitheliasin; FLJ41954; NP_001128571.1; NP_001369649.1; NP_005647.3; PP9284; PRSS10; Serine protease 10; TMPRSS2; transmembrane protease serine 2; transmembrane protease, serine 2