Human TL1A/TNFSF15 Antibody

Catalog #: AF744
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF744
AF744-SP

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All TL1A/TNFSF15 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TL1A/TNFSF15 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TL1A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant mouse TL1A is observed, and less than 2% cross‑reactivity with recombinant human (rh) APRIL, rhTNF-alpha, and rhFas Ligand is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TL1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TL1A/TNFSF15 (Catalog # 1319-TL)

Immunohistochemistry

3-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TL1A/TNFSF15 in Human Prostate. TL1A/TNFSF15 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human TL1A/TNFSF15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF744) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TL1A/TNFSF15

TL1A is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNF superfamily member 15 (TNFSF15). Human TL1A is a 251 aa protein consisting of a 35 aa cytoplasmic domain, a 24 aa transmembrane region and a 192 aa C-terminal extracellular domain. It is a longer variant of the previously cloned TL1 (also known as VEGI) that is possibly a cloning artifact. TL1A is predominantly expressed in endothelial cells and its expression is inducible by TNF-alpha and IL-1 alpha. TL1A binds with high affinity to death receptor 3 (DR3), which is now designated TNF receptor superfamily member 25 (TNFRSF25). DR3 was formerly designated TNFRSF12 when it was thought to be the receptor for TWEAK/TNFSF12. DR3 is expressed primarily on activated T cells. Depending on the cell context, ligation of DR3 by TL1A can trigger one of two signaling pathways, activation of the transcription factor NF-kappa-B or activation of caspases and apoptosis. On primary T cells, TL1A induces NF-kappa-B activation and a costimulatory signal to increase IL-2 responsiveness and the secretion of proinflammatory cytokines. However, in a tumor cell line, TF-1, TL1A has been shown to induce caspase activity and apoptosis. These effects of TL1A are blocked by the secreted, soluble decoy receptor 3 (DcR3), also known as TR6 and TNFRSF6B, which compete with DR3 for binding to TL1A. Consistent with the observed in vitro activities, TL1A promotes ex vivo splenocyte expansion and enhances in vivo graft-versus-host-response.

References

Migone, T.S. et al. (2002) Immunity 16:479.

Long Name

TNF-like 1

Entrez Gene IDs

9966 (Human); 326623 (Mouse)

Alternate Names

MGC129934; MGC129935; TL1A; TL1Vascular endothelial cell growth inhibitor; TNF superfamily ligand TL1A; TNFSF15; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand superfamily member 15; vascular endothelial growth inhibitor-192A; VEGI; VEGI192A; VEGITNF ligand-related molecule 1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human TL1A/TNFSF15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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The somatostatin analogue octreotide inhibits growth of small intestine neuroendocrine tumour cells.
Authors: Li S, Martijn C, Cui T, Essaghir A, Luque R, Demoulin J, Castano J, Oberg K, Giandomenico V
PLoS ONE, 2012-10-31;7(10):e48411.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Two Functionally Distinct Isoforms of TL1A (TNFSF15) Generated by Differential Ectodomain Shedding
Authors: Christoph Muck, Dietmar Herndler-Brandstetter, Lucia Micutkova, Beatrix Grubeck-Loebenstein, Pidder Jansen-Durr
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences