Human TIM-4 Biotinylated Antibody

Catalog #: BAF2929 Datasheet / COA / SDS

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BAF2929

All TIM-4 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TIM-4 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TIM-4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) TIM-1, recombinant mouse (rm) TIM-2, rhTIM-3, rmTIM-4, rmTIM-5, and rmTIM-6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIM-4
Glu25-Leu315
Accession # Q96H15

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TIM‑4 (Catalog # 2929-TM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-4

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1‑3). The human TIM-4 cDNA encodes a 378 amino acid (aa) precursor that includes a 24 aa signal sequence, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1 or TIM-3. Within the ECD, human TIM-4 shares 35% and 23% aa sequence identity with TIM-1 and TIM-3, respectively. A TIM-2 ortholog has not been identified in human. The ECD of human TIM-4 shares 45% aa sequence identity with that of mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (5‑7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4⁺ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells (5).

References

Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494.
Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Feigelstock, D. et al. (1998) J. Virol. 72:6621.
McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
Khademi, M. et al. (2004) J. Immunol. 172:7169.

Long Name

T Cell Immunoglobulin Mucin-4

Entrez Gene IDs

91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)

Alternate Names

FLJ27515; Smuckler; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell membrane protein 4; TIM4; TIM-4; TIMD4; TIMD-4