Human TGF-beta RI/ALK-5 Antibody Summary
Leu34-Glu125
Accession # P36897
Applications
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human TGF‑ beta RI/ALK‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RI/ALK-5
TGF-beta RI, also called ALK-5, is an approximately 55 kDa type I transmembrane serine/threonine receptor kinase (1, 2). It contains a cysteine-rich extracellular domain (ECD), a transmembrane helix, and a C-terminal cytoplasmic kinase domain (3). Within the cytoplasmic domain there is also a short, conserved regulatory sequence known as the GS region that is N-terminal to the kinase domain (1). Within the ECD, human TGF-beta RI shares 90% and 88% aa sequence identity with mouse and rat TGF-beta RI, respectively. In the presence of TGF-beta, TGF-beta RI forms a complex with, and is phosphorylated by, TGF-beta RII (1). Phosphorylated TGF-beta RI can then transiently bind and phosphorylate Smad2 and Smad3 (2, 4-6). These phosphorylated Smads form heteromeric complexes with Smad4, translocate to the nucleus, and regulate target gene transcription (2, 4-6). TGF-beta RI is likely important during development, since mice deficient for TGF-beta RI die at midgestation with severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells (7). Furthermore, TGF-beta RI appears to be involved in proper lymphatic network development (8). Mutations in TGF-beta RI have been identified in pancreatic, colorectal, ovarian, and head and neck cancers (9).
- Wrana, J.L. et al. (1994) Nature 370:341.
- Massagué, J. (2012) Nat. Rev. Mol. Cell Biol. 13:616.
- Huse, M. et al. (1999) Cell 96:425.
- Marcías-Silva, M. et al. (1996) Cell 87:1215.
- Zhang, Y. et al. (1996) Nature 383:168.
- Huse, M. et al. (2001) Mol. Cell 8:671.
- Larsson, J. et al. (2001) EMBO J. 20:1663.
- James, J.M. et al. (2013) Development 140:3903.
- Sheen, Y.Y. et al. (2013) Biomol. Ther. (Seoul) 21:323.
Product Datasheets
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