Human TCL1B Antibody

Catalog #: AF6794 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6794
AF6794-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TCL1B Antibody Summary

Species Reactivity

Human

Specificity

Detects human TCL1B in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human TCL1A is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TCL1B
Ala2-Asp128 (Glu93Arg)
Accession # O95988

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TCL1B in Human Lymphoma. TCL1B was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human TCL1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6794) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCL1B

TCL1B (T cell leukemia protein 1B; also SYN1 and [p15]TML1) is a monomeric,15 kDa (predicted) member of the TCL1 family of proteins. It is expressed in the embryo and syncytiotrophoblast, and interacts with Akt1 and Akt2 kinase, promoting their activation and the subsequent deactivation of proapoptotic molecules. Human TCL1B is 128 amino acids (aa) in length. There is no definitive structural motif that characterizes TCL1B, save for a unique beta -barrel configuration. Full length human TCL1B shares less than 35% aa identity with five orthologous mouse TCL1B isoforms.

Long Name

T Cell Leukemia Protein 1B

Entrez Gene IDs

9623 (Human)

Alternate Names

MTCP1-like protein 1; Oncogene TCL1B; Oncogene TCL-1B; SYN-1; Syncytiotrophoblast-specific protein; T-cell leukemia/lymphoma 1B; T-cell leukemia/lymphoma protein 1B; T-cell lymphoma/leukemia 1B; TCL1; TCL1/MTCP1-like protein 1; TCL1B; TML1; TML1TCL1/ MTCP1-like 1