Human TAFA2/FAM19A2 Antibody

Catalog #: AF4179 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4179
AF4179-SP

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Human TAFA2/FAM19A2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TAFA2/FAM19A2 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human TAFA5 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TAFA2/FAM19A2
Ala31-His131
Accession # Q8N3H0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize the the enhancemment of neurite outgrowth of cortical neurons from E16-E18 rat embryos induced by TAFA-2. The Neutralization Dose (ND50) is typically 5 µg/mL in the presence of 10 µg/mL Recombinant Human TAFA-2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TAFA2/FAM19A2 in Human Brain. TAFA2/FAM19A2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human TAFA2/FAM19A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4179) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAFA2/FAM19A2

TAFA2 (also FAM19A2) is a secreted, 11 kDa member of the FAM19/TAFA family of chemokine-like proteins (1). It is synthesized as a 131 amino acid (aa) precursor that contains a 30 aa signal sequence and a 101 aa mature chain. Like other members of the FAM19/TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern CX₇CCX₁₃CXCX₁₄CX₁₁CX₄CX₅CX₁₀C, where C represents a conserved cysteine residue and X represents any noncysteine amino acid (1). Human TAFA2 is 97% aa identical to mouse TAFA2 (1). TAFA2 expression can be detected in the central nervous system (CNS), colon, heart, lung, spleen, kidney, and thymus, but its expression in the CNS is 50- to 1000-fold higher than in other tissues (1). Within the CNS, TAFA2 expression is highest in the occipital and frontal cortex (3- to 10-fold more abundantly expressed than in other cortical regions) and medulla (1). The biological functions of TAFA family members remain to be determined, but there are a few tentative hypotheses. First, TAFAs may modulate immune responses in the CNS by functioning as brain-specific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). Second, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1 - 2). Finally, TAFAs may control axonal sprouting following brain injury (1).

References

Tang, Y.T. et al. (2004) Genomics 83:727.
Benveniste, E. (1998) Cytokine Growth Factor Rev. 9:259.

Long Name

Family with Sequence Similarity 19, Member A2

Entrez Gene IDs

338811 (Human); 268354 (Mouse)

Alternate Names

Chemokine-like protein TAFA-2; DKFZp761E1217; DKFZp781P0552; FAM19A2; family with sequence similarity 19 (chemokine (C-C motif)-like), member A2; MGC42403; TAFA2; TAFA-2