Human T-bet/TBX21 PerCP-conjugated Antibody Summary
Glu326-Asn535
Accession # Q9UL17
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of T‑bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human T-bet/TBX21 PerCP-conjugated Monoclonal Antibody (Catalog # IC5385C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Intracellular Molecules.
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Detection of T‑bet/TBX21 in CD45RO+/CD8+ PBMC lymphocytes by Flow Cytometry CD45RO+/CD8+ PBMC lymphocytes were stained with Mouse Anti-Human CD8 alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB1509A), Mouse Anti-Human CD45RO PE‑conjugated Monoclonal Antibody (Catalog # FAB10642P), and either (A) Mouse Anti-Human T‑bet/TBX21 PerCP‑conjugated Monoclonal Antibody (Catalog # IC5385C) or (B) isotype control antibody (Catalog # IC002C). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: T-bet/TBX21
T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.
Product Datasheets
Citation for Human T-bet/TBX21 PerCP-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Lipopolysaccharide-Induced Chorioamnionitis Promotes IL-1-Dependent Inflammatory FOXP3+ CD4+ T Cells in the Fetal Rhesus Macaque
Authors: CM Rueda, P Presicce, CM Jackson, LA Miller, SG Kallapur, AH Jobe, CA Chougnet
J Immunol, 2016-04-01;0(0):.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Flow Cytometry
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