Human T-bet/TBX21 Antibody Summary
Glu326-Asn535
Accession # Q9UL17
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of T‑bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB5385, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with ice-cold methanol.
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Detection of Human T-bet/TBX21 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for T-bet/TBX21 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Detection of T‑bet/TBX21 in CD45RO+/CD8+ PBMC lymphocytes by Flow Cytometry CD45RO+/CD8+ PBMC lymphocytes were stained with either (A) Mouse Anti-Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) or (B) isotype control antibody (Catalog # MAB002) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). Cells were then stained with Mouse Anti-Human CD8 alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB1509A) and Mouse Anti-Human CD45RO PE‑conjugated Monoclonal Antibody (Catalog # FAB10642P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: T-bet/TBX21
T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.
Product Datasheets
Citations for Human T-bet/TBX21 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Clinical Use of Propranolol Reduces Biomarkers of Proliferation in Gastric Cancer
Authors: Hu Q, Liao P, Li W et al.
Frontiers in Oncology
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The effect of adalimumab on key drivers in the pathogenesis of psoriasis.
Authors: Hendriks A, van der Velden H, Wolberink E, Seyger M, Schalkwijk J, Zeeuwen P, de Jong E, Pasch M, van Erp P, van de Kerkhof P
Br J Dermatol, 2014-03-01;170(3):571-80.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
TLR2 Stimulation Drives Human Naive and Effector Regulatory T Cells into a Th17-Like Phenotype with Reduced Suppressive Function.
Authors: Nyirenda MH, Sanvito L, Darlington PJ
J. Immunol., 2011-07-20;187(5):2278-90.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Regional immunity in melanoma: immunosuppressive changes precede nodal metastasis.
Authors: Mansfield AS, Holtan SG, Grotz TE
Mod. Pathol., 2010-12-10;24(4):487-94.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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