Human Syndecan-1/CD138 Biotinylated Antibody Summary
Gln18-Glu251
Accession # NP_002988
Applications
Human Syndecan-1/CD138 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Syndecan‑1/CD138 in Human Liver. Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of Human Liver using Goat Anti-Human Syndecan‑1/CD138 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2780) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syndecan-1/CD138
Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1.
Product Datasheets
Citation for Human Syndecan-1/CD138 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Multiple receptor interactions trigger release of membrane and intracellular calcium stores critical for herpes simplex virus entry.
Authors: Cheshenko N, Liu W, Satlin LM, Herold BC
Mol. Biol. Cell, 2007-06-06;18(8):3119-30.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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