Human SRPK3 Antibody

Catalog #: MAB72301 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB72301
MAB72301-SP

Detection of Human SRPK3 by Western Blot.

2 Images

All SRPK3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SRPK3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SRPK3 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 722516

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SRPK3
Arg247-Ser316
Accession # Q9UPE1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human SRPK3 by Western Blot. Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SRPK3 Monoclonal Antibody (Catalog # MAB72301) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SRPK3 at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

View Larger

SRPK3 in Human Skeletal Muscle. SRPK3 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human SRPK3 Monoclonal Antibody (Catalog # MAB72301) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SRPK3

SRPK3 (Ser/Arg-rich protein specific kinase 3), also called MSSK-1 (muscle-specific serine kinase 1) or STK23 (Ser/Thr kinase 23) is a 59 kDa (predicted) cytoplasmic member of the CMGC Ser/Thr protein kinase family. SRPK3 is principally expressed in heart and skeletal muscle. SRPKs selectively phosphorylate Ser on RS domain-containing proteins, initiating mRNA splicing and maturation. The 533 amino acid (aa) human SRPK3 contains a split kinase domain (aa 79‑292 and 347‑531) separated by a hinge region. A 491 aa isoform shows an alternate translation start site at aa 43. Within the region used as an immunogen, human SRPK3 shares 87% and 86% aa identity with mouse and rat SRPK3, respectively.

Long Name

Ser/Arg-rich Protein-specific Kinase 3

Entrez Gene IDs

26576 (Human); 56504 (Mouse); 293854 (Rat)

Alternate Names

MGC102944; MSSK1; MSSK-1; MSSK1EC 2.7.11.1; Muscle-specific serine kinase 1; serine arginine rich protein-specific kinase 3; Serine/arginine-rich protein-specific kinase 3; serine/threonine kinase 23; Serine/threonine-protein kinase 23; serine/threonine-protein kinase SRPK3; SFRS protein kinase 3; SRPK3; SR-protein-specific kinase 3; SRSF protein kinase 3; STK23