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Human SR-AI/MSR1 Antibody

Catalog #: MAB27081
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB27081
MAB27081-SP
Product Details
Citations (4)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SR-AI/MSR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SR-AI in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant mouse SR‑AI.
Source
Monoclonal Mouse IgG1 Clone # 351620
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SR-AI
Lys77-Leu451
Accession # P21757
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human SR-AI/MSR1 (Catalog # 2708-MS)
under non-reducing conditions only
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.01 - 0.05 μg/mL of this antibody will block 50% of the binding of 400 ng/mL of biotinylated AGE-BSA to immobilized recombinant human SR-AI chimera coated at 5 μg/mL (100 µL/well). At 2 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SR-AI/MSR

The type I class A macrophage scavenger receptor (SR-AI; also MSR-AI) is a 70-80 kDa protein that belongs to an ever expanding family of transmembrane molecules collectively referred to as scavenger receptors (1 - 3). Receptors of this family contain characteristic extracellular domains and bind to a series of generally unrelated, but negatively-charged/polyanionic ligands (1, 3). Human SR-AI is a type II transmembrane glycoprotein that is 451 amino acids (aa) in length. It contains a 50 aa cytoplasmic tail, a 26 aa transmembrane segment and a 375 aa extracellular region (4, 5). The extracellular region contains four definitive domains, with a membrane proximal spacer of 33 aa, an alpha -helical coiled-coil domain of 163 aa, a collagen-like domain of 69 aa, and a cysteine-rich C-terminus of 110 aa (4, 6). The cysteine-rich domain (CRD) forms three intrachain disulfide bonds (7). The functional form of the molecule is a 220 - 230 kDa membrane-associated trimer that, in human, apparently has two disulfide bonded chains and a third noncovalently associated subunit (8, 9). Human extracellular region is 73% and 72% aa identical to bovine and mouse SR-AI extracellular region, respectively. The human gene for SR-A gives rise to three isoforms; the I isoform of 451 aa, the II isoform of 358 aa, and the III isoform of 388 aa (4, 5, 10). All are equal through the first 344 aa which includes the cytoplasmic tail through the collagenous domain. Isoform II (SR-AII) shows a severe truncation of the CRD, but is expressed on the cell surface. Isoform III (SR-AIII) has a modest truncation of the CRD, and cannot be expressed on the cell surface. Their functions are unknown. However, relative to SR-AI, SR-AII is known to show differential sensitivity to LPS and receptor binding to gram-negative bacteria (9, 11), while SR-AIII is known to be a dominant-negative isoform (10). SR-AIII may achieve this by either heterotrimerizing with SR-AI, or simply eliminating the production of SR-AI mRNA.

References
  1. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
  2. Linton, M.F. and S. Fazio (2001) Curr. Opin. Lipidol. 12:489.
  3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
  4. Matsumoto, A. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9133.
  5. Emi, M. et al. (1993) J. Biol. Chem. 268:2120.
  6. Naito, M. et al. (1992) Am. J. Pathol. 141:591.
  7. Resnick, D. et al. (1996) J. Biol. Chem. 271:26924.
  8. Ashkenas, J. et al. (1993) J. Lipid Res. 34:983.
  9. Penman, M. et al. (1991) J. Biol. Chem. 266:23985.
  10. Gough, P.J. et al. (1998) J. Lipid Res. 39:531.
  11. Peiser, L. et al. (2000) Inf. Immun. 68:1953.
Long Name
Macrophage Scavenger Receptor Types I and II
Entrez Gene IDs
4481 (Human); 20288 (Mouse); 25073 (Rat)
Alternate Names
CD204 antigen; CD204; Macrophage acetylated LDL receptor I and II; macrophage scavenger receptor 1; macrophage scavenger receptor type III; MSR1; phSR1; phSR2; SCARA1; SCARA1macrophage scavenger receptor types I and II; Scavenger receptor class A member 1; scavenger receptor class A, member 1; SR-A; SRAI; SR-AI

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Citations for Human SR-AI/MSR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Class A1 scavenger receptor antibody improves murine colitis by influencing macrophage and gut microbiota
    Authors: Su, J;Liu, L;Ren, Y;Gan, Y;Lin, Y;Xie, C;
    Scientific reports
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Human Scavenger Receptor A1-Mediated Inflammatory Response to Silica Particle Exposure Is Size Specific
    Authors: N Nishijima, T Hirai, K Misato, M Aoyama, E Kuroda, KJ Ishii, K Higashisak, Y Yoshioka, Y Tsutsumi
    Front Immunol, 2017-04-03;8(0):379.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. CXCR4 positive and angiogenic monocytes in myocardial infarction.
    Thromb Haemost, 2012-12-06;109(2):255-62.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  4. Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
    Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
    J. Thromb. Haemost., 2011-05-01;9(5):1056-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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