Human SOX9 NorthernLights™ NL557-conjugated Antibody

Catalog #: NL3075R
1 Citation Datasheet / COA / SDS

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NL3075R

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All SOX9 Antibodies

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Human SOX9 NorthernLights™ NL557-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SOX9 in direct ELISAs and Western blots. In these formats, approximately 10% cross-reactivity with recombinant human (rh) SOX10 is observed and less than 5% cross-reactivity with rhSOX2, rhSOX3, rhSOX7, and rhSOX17 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SOX9
Met1-Lys151
Accession # P48436

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

NL557 (Excitation= 557 nm, Emission= 574 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-15 µg/mL

Immersion fixed U-251 MG human glioblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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SOX9 in U‑251 MG Human Cell Line. SOX9 was detected in immersion fixed U‑251 MG human glioblastoma cell line using Goat Anti-Human SOX9 NorthernLights™ NL557-conjugated Antigen Affinity-purified Polyclonal Antibody (red, Catalog # NL3075R) at 5 µg/mL for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: SOX9

SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

Long Name

Transcription Factor SOX9

Entrez Gene IDs

6662 (Human)

Alternate Names

campomelic dysplasia, autosomal sex-reversal; CMD 1; CMD1; CMPD1; SOX9; SRA1SRY (sex-determining region Y)-box 9 protein; SRY (sex determining region Y)-box 9; SRY-related HMG-box, gene 9; transcription factor SOX-9

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human SOX9 NorthernLights™ NL557-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1